银杏叶黄酮类化合物水提工艺的响应面优化

研究水浸提银杏叶中总黄酮类物质的最佳工艺条件.以银杏粉碎叶为试验材料,以水为提取溶剂,采用响应面设计方法,对影响黄酮提取效果的提取温度、提取时间和料液比等3个因素先后进行了部分因子试验和中心组合设计试验,并建立数学模型,研究这些因素对黄酮提取率的影响.部分因子试验结果表明:提取时间和料液比是影响仙人掌黄酮提取效果的主要因子;中心组合设计试验建立的黄酮提取率(Y1)与提取温度(X1)、提取时间(X2)、料液比(X3)间的数学模型为Y1=6.423 667+0.161*X1+0.684 25*X2-0.523 75*X3-0.660 083*X1*X1+0.174 5*X1*X2-0.657 5*X1*X3-0.534 583*X2*X2-0.013 5*X2*X3-1.527 583*X3*X3;最佳的提取工艺条件为料液比1∶13.76,提取时间3.70 h,提取温度93.37℃,该条件下模型预测的最大提取率为6.75%.结果表明应用响应面方法对黄酮类物质的提取条件进行优化是非常有效的.

OPTIMIZATION ON EXTRACTION CONDITIONS OF FLAVONOIDS FROM GINKGO BILOBA LEAF USING RESPONSE SURFACE METHODOLOGY

The article aim was to study the maximum extraction conditions of flavonoids from Ginkgo biloba by water.With dried Ginkgo biloba powder as material and water as solvent,by using response surface methodology,the effects of extracting temperature,extracting time and ratio of material to solvent were studied,and then the regression model was established to study the extracting condition.The result of fractional factorial design indicated that extracting time and ratio of material to solvent played important roles in flavonoids extracting.The regression model of extraction rate(Y1) to ethanol concentration(X2) and ratio of material to solvent(X3) was Y1 = 6.423 667 + 0.161*X1 + 0.684 25*X2-0.523 75*X3-0.660 083*X1*X1+0.174 5*X1*X2-0.657 5*X1*X3-0.534 583*X2*X2-0.013 5*X2*X3-1.527 583*X3*X3,and the optimum conditions were ratio of material to solvent 1∶13.76,time 3.70 h,and temperature 93.37 ℃.The maximum extraction rate predicted by the model was 6.754%.Final results show that response surface methodology was a good method for optimizing extraction conditions of flavonoids.