| Increase in electron transfer activity in photosystem Ⅱ of spinach thylakoids caused by conversion of phosphatidyl- glycerol to phosphatidic acid molecules |
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| 作者姓名： | WUFeng YANGZhenle LILiangbi KUANGTingyun |
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| 作者单位： | KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiology＆PhotosynthesisResearchCenter,InstituteofBotany,ChineseAcademyofSciences,Beijing100093,China |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划) |
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| 摘 要： | The techniques of oxygen electrode polarography, sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) and thin layer chromatography (TLC) were employed to investigate the effect of phospholipase D treatment on physiological function of spinach thylakoids. It was shown that the phospholipase D treatment on thylakoid resuited in the degradation of phosphatidylglycerol (PG) and occurrence of phosphatidic acid (PA). The changes of PG to PA molecules caused an increase in oxygen evolution in photosystem Ⅱ (PS Ⅱ), which was accompanied by an uncoupling effect on thylakoid membrane. It was revealed that the head-groups of PG molecules play an important role in themaintenance of the appropriate physiological activity of thylakoid membrane.
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| 关 键 词： | 电子转移活性 类囊体 光合作用 菠菜 磷脂酰甘油 |
| 收稿时间： | 2003-03-28 |
Increase in electron transfer activity in photosystem II of spinach thylakoids caused by conversion of phosphatidylglycerol to phosphatidic acid molecules |
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| WUFeng YANGZhenle LILiangbi KUANGTingyun.Increase in electron transfer activity in photosystem II of spinach thylakoids caused by conversion of phosphatidylglycerol to phosphatidic acid molecules[J].Chinese Science Bulletin,2003,48(15):1581-1585. |
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| Authors: | Wu Feng Yang Zhenle Li Liangbi Kuang Tingyun |
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| Affiliation: | e-mail: yangzhl @ns.ibcas.ac.cn |
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| Abstract: | The techniques of oxygen electrode polarography, sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) and thin layer chromatography (TLC) were employed to investigate the effect of phospholipase D treatment on physiological function of spinach thylakoids. It was shown that the phospholipase D treatment on thylakoid resulted in the degradation of phosphatidylglycerol (PG) and occurrence of phosphatidic acid (PA). The changes of PG to PA molecules caused an increase in oxygen evolution in photosystem Ⅱ (PSⅡ), which was accompanied by an uncoupling effect on thylakoid membrane. It was revealed that the head-groups of PG molecules play an important role in the maintenance of the appropriate physiological activity of thylakoid membrane. |
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| Keywords: | thylakoid membrane phospholipase D phosphatidylglycerol phosphatidic acid oxygen evolution |
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