| 小鼠破骨细胞骨髓诱导模型的建立及功能观察 |
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| 引用本文： | 鲁秀敏,苏楠,李福兵,赵玲,陈林.小鼠破骨细胞骨髓诱导模型的建立及功能观察[J].第三军医大学学报,2008,30(6):521-524. |
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| 作者姓名： | 鲁秀敏 苏楠 李福兵 赵玲 陈林 |
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| 作者单位： | 1. 第三军医大学大坪医院野战外科研究所全军战创伤中心,创伤、烧伤与复合伤国家重点实验室,重庆,400042;重庆工学院生物工程学院,重庆,400050
2. 第三军医大学大坪医院野战外科研究所全军战创伤中心,创伤、烧伤与复合伤国家重点实验室,重庆,400042 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金，国家自然科学基金 |
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| 摘 要： | 目的 获得大量高质量小鼠破骨细胞,为体外研究破骨细胞骨吸收功能提供丰富的细胞来源.方法 采用巨噬细胞集落刺激因子(maerophage colony stimulating factor,M-CSF)和破骨细胞分化因子(receptor activator of nuclearfactor-κB ligand,RANKL)诱导小鼠骨髓单核细胞分化为破骨细胞,并通过抗酒石酸酸性磷酸酶(tartrate-resistant acid phos-phatase,TRAP)染色和骨吸收实验来鉴定破骨细胞及其噬骨能力.结果 诱导3 d后可见TRAP( )多核细胞出现,诱导5 d后骨片上可见蓝紫色的吸收陷窝.随着培养时间的延长,TRAP( )多核细胞数目和吸收陷窝呈现时间依赖性增长趋势(P<0.05).结论 M-CSF和RANKL诱导小鼠骨髓单核细胞可产生大量的具有活跃噬骨能力的破骨细胞.
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| 关 键 词： | 破骨细胞 TRAP染色 骨吸收 |
| 文章编号： | 1000-5404(2008)06-0521-04 |
| 修稿时间： | 2007年8月30日 |
Establishment of experimental model of osteoclast differentiation induced by bone marrow mononuclear phagocytes of mouse |
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| LU Xiu-min,SU Nan,LI Fu-bing,ZHAO Ling,CHEN Lin.Establishment of experimental model of osteoclast differentiation induced by bone marrow mononuclear phagocytes of mouse[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(6):521-524. |
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| Authors: | LU Xiu-min SU Nan LI Fu-bing ZHAO Ling CHEN Lin |
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| Affiliation: | 1State Key Laboratory of Trauma;Burns and Combined Injury;Trauma Center;Institute of Surgery Research;Daping Hospital;Third Military Medical University;Chongqing 400042;2College of Bioengineering;Chongqing Institute of Technology;Chongqing 400050;China |
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| Abstract: | Objective To obtain plenty of pure osteoclasts for researching the function of osteoclasts in vitro. Methods Osteoclasts were induced by bone marrow mononuclear phagocytes of mouse using medium with macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-kappaB ligand (RANKL). The tartrate resistant acid phosphatase (TRAP) staining and bone resorption experiment were used to identify the osteoclasts. Results The TRAP positive multinuclear cells were induced from mouse bone marr... |
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| Keywords: | osteoclast tartrate resistant acid phosphatase bone resorption |
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