| 钛表面固定纤维连接蛋白及其体外细胞培养 |
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| 引用本文： | 饶桦强,冯波,鲁雄,汪建新,江旭,翁杰.钛表面固定纤维连接蛋白及其体外细胞培养[J].复合材料学报,2008,25(6):67-71. |
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| 作者姓名： | 饶桦强 冯波 鲁雄 汪建新 江旭 翁杰 |
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| 作者单位： | 西南交通大学 材料先进技术教育部重点实验室, 成都 610031 |
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| 基金项目： | 国家自然科学基金资助项目(30370408); 教育部全国博士学位论文作者专项资金资助项目（200554） |
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| 摘 要： | 通过低温等离子聚合的方法，以丙烯酸为单体在钛表面沉积含有羧基的薄膜，以羧基为接入点固定纤维连接蛋白。样品表面用X射线光电子能谱、傅里叶红外光谱仪进行表征。将固定了纤维连接蛋白的样品进行体外细胞培养，所用的细胞为MG63骨瘤细胞，对照样为纯钛。结果表明， 钛表面聚丙烯酸薄膜能有效地固定纤维连接蛋白，并且固定纤维连接蛋白的样品能促进骨瘤细胞的生长和黏附，具有更高的成骨活性。
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| 关 键 词： | 钛 丙烯酸 纤维连接蛋白 低温等离子聚合 细胞培养 |
| 收稿时间： | 2008-03-06 |
| 修稿时间： | 2008-06-16 |
Immobilization of fibronectin on titanium and cell culture |
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| RAO Huaqiang,FENG Bo,LU Xiong,WANG Jianxin,JIANG Xu,WENG Jie.Immobilization of fibronectin on titanium and cell culture[J].Acta Materiae Compositae Sinica,2008,25(6):67-71. |
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| Authors: | RAO Huaqiang FENG Bo LU Xiong WANG Jianxin JIANG Xu WENG Jie |
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| Affiliation: | Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China |
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| Abstract: | The low temperature plasma polymerization was used to introduce carboxyl (—COOH) onto the titanium surface. Fibronectin was immobilized to the surface via —COOH. The surface components were characterized by X-ray photoelectron spectroscopy and Fourier transform infrared reflection spectroscopy. The MG63 cells were seeded on the surface of the modified samples and the pure titanium was used as control. The results indicate that fibronectin is immobilized on the titanium and the modified samples are beneficial for the growth and adherence of the cells. The osteogenic activity of the samples with immobilized fibronectin is better than that of the pure titanium. |
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| Keywords: | titanium crylic acid fibronectin low temperature plasma polymerization cell culture |
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