| siRNA沉默HER2对人胰腺癌细胞PANC-1侵袭能力的影响 |
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| 引用本文： | 公伟,;梁飞,;刘军,;李春友,;杨凤辉,;郑顺贞,;李光兵.siRNA沉默HER2对人胰腺癌细胞PANC-1侵袭能力的影响[J].中国现代普通外科进展,2014(9):678-682. |
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| 作者姓名： | 公伟 ;梁飞 ;刘军 ;李春友 ;杨凤辉 ;郑顺贞 ;李光兵 |
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| 作者单位： | [1]山东大学附属省立医院器官移植、肝胆外二科,山东济南250021; [2]山东大学附属省立医院乳腺甲状腺外科,山东济南250021 |
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| 基金项目： | 山东省医药卫生科技发展计划（2009HW064） |
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| 摘 要： | 目的:探讨慢病毒介导的siRNA沉默HER2对人胰腺癌细胞株PANC-1侵袭能力的影响。方法:运用HER2基因小干扰RNA(siRNA)的重组慢病毒稳定转染PANC-1细胞,荧光定量RT-PCR和Western blotting观察HER2 mRNA和蛋白表达的改变,体外侵袭实验检测细胞侵袭能力的变化。结果:HER2基因小干扰RNA的重组慢病毒稳定转染PANC-1细胞可显著抑制HER2 mRNA和蛋白表达;体外侵袭实验显示siRNA沉默HER2后,PANC-1细胞侵袭能力明显下降。结论:HER2基因小干扰RNA的重组慢病毒能有效抑制HER2的表达,抑制胰腺癌细胞体外侵袭能力。siRNA沉默HER2可能为预防和治疗胰腺癌的侵袭转移提供一种新的策略。
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| 关 键 词： | 胰腺肿瘤 RNA干扰 HER2 侵袭 |
Effect of siRNA-mediated HER2 gene silencing on the invasiveness of human pancreatic cancer PANC-1 cell |
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| Affiliation: | GONG Wei, LIANG Fei, LIU Jun, LI Chun-you, YANG Feng-hui, ZHENG Shun-zhen, LI Guang-bing(1 Department of Organ Transplantation and Second Hepatobilary Surgery, 2 Department of Mammary Gland Thyroid Surgery, Provincial Hospital Affiliated to Shandong Uni- versity( Jinan 250021, China) |
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| Abstract: | Obective： To investigate the effect of siRNA-mediated HER2 gene silencing on the invasiveness of human pancretic cancer PANC-1 cell. Metihods： The lentivirus of small interfer- ence RNA targeting HER2 gene was stably transfected to PANC-1 cell. HER2 mRNA and protein expression were examined using fluorescence quantitative PCR and Western blotting respectively.The invasion abilitv of PANC-1 cell was detennined by cell invasion assay in vitro Results： mRNA and protein expression of HER2 were inhibited significantly by stable transfection of the lentivirus of siRNA targeting HER2 gene HER2 silence with siRNA significantly inhibited the invasion ability of PANC-1 cell, Conclusion： HER2 silence with siRNA significantly inhibits the expression of HER2 and the invasion ability of pancreatic cancer PANC-1 cell, |
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| Keywords: | Pancretic cancer- RNA interence- Invasion·Metastasis |
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