通心络对兔外周血管球囊损伤后血栓形成及内膜增生的影响

目的 观察中成药通心络对兔腹主动脉及骼动脉球囊损伤后血栓形成及内膜增生的影响。方法 新西兰兔36只, 随机分为3组:通心络组16只, 阿司匹林组11只, 安慰剂组9只。各组服药剂量: 通心络0.38 g·kg^-1·d^-1, 阿司匹林50 mg·kg^-1·d^-1, 安慰剂组服用生理盐水10 ml·d^-1。1 w k 后制作腹主动脉和骼动脉球囊损伤模型, 在模型制作后24h、1 w k 或1 mon 取受损节段血管, 经HE 染色后置显微镜下观察管腔内血栓形成及内膜增生情况。结果 球囊损伤后24 h、7 d 在各实验组均可见部分血管腹主动脉及髂动脉内膜增生, 管腔狭窄5 %~40 %, 术后1 mon 安慰剂组2 只兔腹主动脉及髂动脉内膜增生更为显著, 管腔狭窄50 %~70 %。阿司匹林组血栓形成的血管比例少于另两组, 但无显著差异。通心络组出现内膜增生的血管比例显著少于阿司匹林及安慰剂组。结论 兔腹主动脉和髂动脉球囊损伤模型是研究PTCA 术后再狭窄的理想模型。通心络明显抑制兔外周血管球囊损伤后内膜增生, 提示通心络在再狭窄防治中可能有一定的潜在价值。

Effect of herbal tongxinluo on thrombus formation and neointimal hyperplasia of the balloon injured rabbit peripheral arteries

Aim The effect of herbal tongxinluo on the the balloon injured rabbit abdominal aorta and il- thrombus formation and neointimal hyperplasia of iac artery was observed. Methods 36 New zealand rabbits were divided randomly into 3 groups: tongxinluo group (16 rabbits); aspirin group (11 rabbits) and control group (9 rabbits). The rabbits were treated with herb tongxinluo 0.38 g mg·kg^-1·d^-1, aspirin 50 mg·kg^-1·d^-1 and 0.09% natrium chloride 10 ml·d^-1 alternatively. The arterial injury model was made 1 week after taking the medicine and the injured segments were taken at 24 hours, 1 week or 1 month after the procedure,then the samples were stained by hematoxylin and eosin. The thrombus formation and the neointimal hyperplasia of the injured arteries were observed under microscope. Results The arterial neointimal hyperplasia was seen in both abdominal aorta and iliac artery 24 hours and 7 days after the balloon injury in each group,which led to stenosis in 5%~40% of the arterial lumen, the neointimal hyperplasia was much more significant in 2 rabbits 1 month after the balloon injury. The ratio of the arteries with thrombosis in aspirin group was less than that in the other 2 groups, but the difference was not significant. The ratio of neointimal hyperplasia in tongxinluo group was significantly less than that in aspirin and control groups. Conclusion The balloon injured rabbit abdominal aorta and iliac artery appeare to be an ideal model in researching restenosis after percutaneous transluminar coronary angioplasty (PTCA). Herbal Tongxinluo significantly inhibit the neointimal hyperplasia of the balloon injured rabbit peripheral arteries, which indicate that herbal Tongxinluo might be a promising medicine in preventing restenosis.