| 北京地区腹泻病患者致泻性大肠埃希菌感染类型及其流行特征 |
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| 引用本文： | 曲梅,张新,钱海坤,吕冰,黄瑛,严寒秋,梁志超,贾蕾,王全意.北京地区腹泻病患者致泻性大肠埃希菌感染类型及其流行特征[J].中华流行病学杂志,2014,35(10):1123-1126. |
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| 作者姓名： | 曲梅 张新 钱海坤 吕冰 黄瑛 严寒秋 梁志超 贾蕾 王全意 |
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| 作者单位： | 100013,北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室 |
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| 基金项目： | 北京市高层次卫生技术人才培养计划(2013-3-099)；国家“十二五”科技重大专项(2012ZX10004215-003-001)This work was supported by grants from the Project of Beijing High-Level Technical Personnel Training in Health，National Science and Technology Support Projects for the“Twelfth Five-Year Plan”for Infectious Diseases of China |
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| 摘 要： | 目的：了解北京地区腹泻人群中致泻性大肠埃希菌（DEC）毒力基因的分布及流行特征。方法对2012-2013年监测医院的腹泻患者粪便样本进行分离培养，再采用实时荧光PCR检测毒力基因。结果6370份粪便标本有253份阳性，共分离到DEC 262株，阳性检出率为4.0%（253/6370），9份标本发现两种不同型别的DEC混合感染。肠致病性大肠埃希菌（EPEC）为42.8%，其中非典型EPEC占42.0%，典型EPEC占0.8%；肠产毒性大肠埃希菌（ETEC）为38.9%，其中耐热肠毒素基因st阳性占24.8%，不耐热型肠毒素基因lt阳性占9.9%，st和lt均阳性占4.2%；肠聚集性大肠埃希菌（EAEC）为15.3%；肠侵袭性大肠埃希菌（EIEC）为2.7%；检出1株产志贺毒素大肠埃希菌（STEC），血清型为O26∶K60。ETEC感染具有明显的年龄分布特征；各型DEC全年均有检出，且呈季节性变化。结论北京地区腹泻病患者DEC感染类型以EPEC和ETEC为主，且EPEC分离株多为非典型，并存在混合感染，不同型别DEC感染具有年龄和季节性分布特征。
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| 关 键 词： | 致泻性大肠埃希菌 毒力基因 实时荧光PCR |
| 收稿时间： | 2014/5/26 0:00:00 |
Study on the genotype and epidemic characteristics of diarrheagenic Escherichia coli isolated from diarrheal patients in Beijing |
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| Qu Mei,Zhang Xin,Qian Haikun,Lyu Bing,Huang Ying,Yan Hanqiu,Liang Zhichao,Jia Lei and Wang Quanyi.Study on the genotype and epidemic characteristics of diarrheagenic Escherichia coli isolated from diarrheal patients in Beijing[J].Chinese Journal of Epidemiology,2014,35(10):1123-1126. |
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| Authors: | Qu Mei Zhang Xin Qian Haikun Lyu Bing Huang Ying Yan Hanqiu Liang Zhichao Jia Lei and Wang Quanyi |
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| Affiliation: | Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China |
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| Abstract: | |
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| Keywords: | Diarrheagenic Escherichia coli Virulence gene Real-time PCR |
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