Comparison of chlorophyll a spectra in wild-type and mutant barley chloroplasts grown under day or intermittent light |
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Authors: | Jeanette S Brown Siegrid Schoch |
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Affiliation: | (1) Carnegie Institution of Washington, 94305 Stanford, CA, USA;(2) Present address: Botanisches Inst. der Univ. Munchen, Menzinger Str. 67, D-8000, Munchen 19, FRG |
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Abstract: | The absorption (640–710 nm) and fluorescence emission (670–710 nm) spectra (77 K) of wild-type and Chl b-less, mutant, barley chloroplasts grown under either day or intermittent light were analysed by a RESOL curve-fitting program. The usual four major forms of Chl a at 662, 670, 678 and 684 nm were evident in all of the absorption spectra and three major components at 686, 693 and 704 nm in the emission spectra. A broad Chl a component band at 651 nm most likely exists in all chlorophyll spectra in vivo. The results show that the mutant lacks not only Chl b, but also the Chl a molecules which are bound to the light-harvesting, Chl a/b, protein complex of normal plants. It also appears that the absorption spectrum of this antenna complex is not modified appreciably by its isolation from thylakoid membranes.Abbreviations Chl
chlorophyll
- DL
daylight
- ImL
intermittent light
- WT
wildtype
- LHC
light-harvesting Chl a/b protein complex
- S.E.
standard error of the mean
DBP-CIW No. 763. |
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Keywords: | absorption chlorophyll chloroplasts fluorescence photosynthesis spectral curve analysis |
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