| 乳糖酶交联酶聚体的制备及性能 |
| |
| 引用本文： | 王康,王璋,高阳,孟广莹.乳糖酶交联酶聚体的制备及性能[J].化学工程,2012,40(10). |
| |
| 作者姓名： | 王康 王璋 高阳 孟广莹 |
| |
| 作者单位： | 天津大学 化学工程研究所,天津,300072 |
| |
| 基金项目： | 天津市重大支撑计划项目 |
| |
| 摘 要： | 以双醛淀粉(DAS)为交联剂,分别制备了乳酸克鲁维酵母和米曲霉来源的乳糖酶交联酶聚体(CLEAs),同时添加牛血清白蛋白(BSA)作为保护剂以提高CLEAs活性。当DAS质量分数为10%、氧化度为80%、BSA与酶蛋白质量比为1∶8时得到的酵母和曲霉乳糖酶CLEAs的活力保留分别为53.84%和55.25%。CLEAs的最适pH值较游离酶有所降低。曲霉乳糖酶CLEAs在60℃下具有较好的热稳定性,并且在37℃下重复使用5次(20 h)后活力可保留52%。
|
| 关 键 词： | 交联酶聚体 乳糖酶 双醛淀粉 牛血清白蛋白 乳糖水解 |
Preparation and properties of cross-linked enzyme aggregates (CLEAs) of β-galactosidase |
| |
| WANG Kang
,
WANG Zhang
,
GAO Yang
,
MENG Guang-ying.Preparation and properties of cross-linked enzyme aggregates (CLEAs) of β-galactosidase[J].Chemical Engineering,2012,40(10). |
| |
| Authors: | WANG Kang WANG Zhang GAO Yang MENG Guang-ying |
| |
| Abstract: | Two kinds of cross-linked enzyme aggregates(CLEAs) of β-galactosidase from Kluyveromyces lactis and Aspergillus oryzae were prepared by using dialdehyde starch(DAS) as cross-linker.Adding bovine serum albumin(BSA) as protective agent was helpful to raise the activity of CLEAs.In the condition of DAS mass fraction 10%,oxidation degree 80% and mass ratio of enzyme to BSA 1 ∶ 8,the obtained activity residues of K.lactis and A.oryzae β-galactosidase CLEAs were 53.84% and 55.25% respectively.The optimum pH values of the two CLEAs were both decreased.A.oryzae β-galactosidase CLEAs presented a high thermal stability at 60 ℃,with its activity retained about 52% after repeated using for 5 cycles(20 h) at 37 ℃. |
| |
| Keywords: | CLEAs β-galactosidase DAS BSA lactose hydrolysis |
| 本文献已被 万方数据 等数据库收录！ |
|
