| rNM23-H1/NDPK-A中试纯化工艺研究 |
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| 引用本文： | 钱垂文,罗勇,张美英,林锋,王一飞.rNM23-H1/NDPK-A中试纯化工艺研究[J].生物技术,2002,12(1):23-24. |
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| 作者姓名： | 钱垂文 罗勇 张美英 林锋 王一飞 |
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| 作者单位： | 广州暨南生物医药研究开发基地,广东, 广州 510632 |
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| 基金项目： | “8 6 3”科研基金项目 (10 2 - 0 8- 0 7- 0 5 )，广州市科委
基金资助项目 (1999-Z - 0 0 7- 0 1) |
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| 摘 要： | 为比较3种DEAE填料对rNM23-H1/NDPK-A的纯化效果，利用同一批中试发酵样品在相同的条件下进行离子交换层析，分别收集P0．2和P1．0两个洗脱峰。通过对洗脱峰中蛋白质含量、目标蛋白相对含量以及酶比活的测定，计算得出Matrex Cellufine A-200,DEAE Sephadex A-25,Macro-Prep DEAE Support三种填料相对应的NDPK－A得率及纯化倍数分别为74．5％、40．8％、92．6％、2．4、1．9、3．1倍。综合分析表明Macro-Prep DEAE Support填料对rNM23-H1/NDPK-A的纯化效果最好。
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| 关 键 词： | rNM23－H1／NDPK－A 填料 纯化工艺 目标蛋白 抑癌基因 |
| 文章编号： | 1004-311X(2002)01-0023-02 |
| 修稿时间： | 2001年8月29日 |
Purification of rNM23-H1/NDPK-A in middle-scale |
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| QIAN Chui-wen,LUO Yong,ZHANG Mei-ying,LIN Feng,WANG Yi-fei.Purification of rNM23-H1/NDPK-A in middle-scale[J].Biotechnology,2002,12(1):23-24. |
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| Authors: | QIAN Chui-wen LUO Yong ZHANG Mei-ying LIN Feng WANG Yi-fei |
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| Abstract: | To compare purification of rNM23-H1/NDPK-A with three DEAE media,ion exchange chromatography experiments were made using a batch of fermentation samples at the same operating conditions Fractions of P 0 2 and P 1 0 were collected solely By analysis of parameters of fractions of P 0 2 and P 1 0 in all experiments,efficient of NDPK-A and purification times of enzyme activity of one microgram protein is 74 5%,40 8%,92 6%,2 4,1 9,3 1 times corresponding to Matrex Cellufine A-200,DEAE Sephadex A-25,Macro-Prep DEAE support media From these above,a conclusion can be drawn that Macro-Prep DEAE support media is the best one in three media |
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| Keywords: | media DEAE purification |
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