MicroRNA-539靶向膜相关的鸟苷酸激酶蛋白1抑制甲状腺癌细胞的侵袭与迁移

目的 观察microRNA-539（miR-539）的表达对甲状腺癌细胞侵袭与迁移的影响，探究miR-539与膜相关的鸟苷酸激酶蛋白1（CARMA1）在甲状腺癌中相互作用的机制。方法 构建miR-539与CARMA1高表达及低表达的细胞模型后通过transwell及MTT法检测两种不同的甲状腺癌细胞的侵袭、迁移及增殖能力。Western blot及RT-PCR检测细胞中蛋白及mRNA表达。结果  miR-539抑制甲状腺癌细胞的侵袭与迁移。通过荧光素酶报告检测发现miR-539通过靶向CARMA1的3’-UTR端从而起到抑制甲状腺癌中CARMA1的作用。进一步的研究证实，CARMA1可显著促进甲状腺癌细胞的侵袭与迁移。而调节CARMA1的表达，miR-539随之变化。研究还发现，与对照组比较，甲状腺癌中miR-539的表达量降低而CARMA1的表达量增加。结论  miR-539靶向CARMA1抑制甲状腺癌细胞的侵袭与迁移。

miR-539 directly targeting CARMA1 inhibits thyroid cancer cell migration and invasion

Objective To observe the impact of miR-539 on the invasion and migration of human thyroid carcinoma, and to investigate the interaction mechanism between miR-539 and caspase recruitment domain-containing membrane-associated guanylate kinase protein 1 (CARMA1). Methods  The ability of cells to migrate and invade was assayed using transwell inserts and MTT assay. The expressions of miR-539 and CARMA1 in thyroid cancer cells were detected by Western blot and RT-PCR. Results  miR-539 inhibited the invasion and migration of thyroid cancer cells. Luciferase reporter assay confirmed that miR-539 binding to the 3''-UTR region of CARMA1 inhibited the expression of CARMA1 in thyroid cancer cells. Further studies demonstrated that CARMA1 significantly promoted the migration and invasion of thyroid cancer cells. miR-539 changed as the expression of CARMA1 was regulated. Furthermore, the expression of miR-539 decreased while the expression of CARMA1 increased in thyroid cancer cell lines and thyroid cancer tissues compared with controls. Conclusions miR-539 can regulate migration and invasion in human thyroid cancer cells by targeting CARMA1.