| 山东花生茎腐病病原菌研究 |
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| 引用本文： | 郭洪参,张悦丽,齐军山,张博,李林,徐作珽,李长松,张广民.山东花生茎腐病病原菌研究[J].中国油料作物学报,2014,36(4):524. |
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| 作者姓名： | 郭洪参 张悦丽 齐军山 张博 李林 徐作珽 李长松 张广民 |
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| 作者单位： | 1. 山东农业大学植物保护学院，山东 泰安，271018；2．山东省农业科学院植物保护研究所/山东省植物病毒重点实验室，山东 济南，250100 |
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| 基金项目： | 山东省现代花生产业技术体系 |
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| 摘 要： | 从山东省多地采集的花生茎腐病株上分离到15个茎腐病菌株，选取6个典型菌株，对病原菌进行形态特征、致病性、生物学特性研究。核糖体DNA-ITS序列分析表明，6个菌株的核糖体DNA-ITS序列同源性高达99%。根据病原菌的形态特征、核糖体DNA-ITS序列同源性比较，确认供试6个菌株为同一种病原菌，与GenBank中可可毛色二孢属（Ｌasiodiplodia）（Diplodia的同物异名）的同源性高达99%以上。根据6个菌株的形态特征、致病性测定结果及生物学特性，并结合核糖体DNA-ITS序列分析，将山东花生茎腐病的病原鉴定为棉色二孢菌（Diplodia gossypina )。
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| 关 键 词： | 花生茎腐病 核糖体rDNA－ITS序列 病原鉴定 |
Research on Pathogen of Peanut Stem Rot in Shandong Province |
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| GUO Hong-can,ZHANG Yue-li,QI Jun-shan,ZHANG Bo,LI Lin,XU Zuo-ting,LI Chang-song,Zhang Guang-min.Research on Pathogen of Peanut Stem Rot in Shandong Province[J].Chinese Journal of Oil Crop Sciences,2014,36(4):524. |
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| Authors: | GUO Hong-can ZHANG Yue-li QI Jun-shan ZHANG Bo LI Lin XU Zuo-ting LI Chang-song Zhang Guang-min |
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| Abstract: | Six representative strains were selected from peanut stem rot plants collected from Shandong Province. Morphological characteristics, pathogenicity and biological characteristics of 6 representative strains were studied. Ribosomal DNA-ITS sequence analysis showed that the sequence homology of these 6 strains was up to 99%. The tested 6 isolates were confirmed as one species. The homology of DNA-ITS sequences of the 6 strains was up to more than 99% compared with Lasiodiplodia spp (synonyms Diplodia) in GenBank. According to morphological characteristics, pathogenicity test and biological characteristics of the 6 tested strains, and combined with the ribosomal rDNA-ITS sequence analysis, the pathogen of peanut stem rot from Shandong Province was identified as Diplodia gossypina. |
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| Keywords: | Peanut collar rot Ribosomal DNA-ITS Pathogen identification |
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