| 人T-bet基因的克隆及序列分析 |
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| 引用本文： | 杨敏,王锁英,王胜军,马洁,毛朝明,邱谷风,许小朋,邵启祥,许化溪.人T-bet基因的克隆及序列分析[J].江苏大学学报(医学版),2006,16(2):109-111,119. |
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| 作者姓名： | 杨敏 王锁英 王胜军 马洁 毛朝明 邱谷风 许小朋 邵启祥 许化溪 |
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| 作者单位： | 1. 江苏大学医学技术学院免疫研究室
2. 江苏大学附属医院儿科,江苏,镇江,212001 |
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| 基金项目： | 中国科学院资助项目;国家自然科学基金 |
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| 摘 要： | 目的：克隆人T-bet基因全长编码区的cDNA，并进行豁定和测序分析，为从转录水平研究Th1／Th2平衡及其与免疫相关性疾病发生发展的关系奠定基础。方法：采用RT-PCR方法，从人外周血淋巴细胞获得T-bet基因的cDNA；连接至pGEM-Teasy载体，导入大肠杆菌DH5α并选择阳性克隆；应用M13F／R通用引物进行双向反应测序，以作序列鉴定。结果：扩增得到的T-bet基因cDNA全长1670 bp，包括编码区1607 bp，编码530个氨摹酸残基，与Genbank中发表的序列完全一致。结论：获得了人T-bet基网的克隆，为进一步研究其生物学功能构建了基础平台，为寻找多种免疫性疾病的有效治疗提供新思路。
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| 关 键 词： | T-bet基因 cDNA克隆 |
| 文章编号： | 1671-7783（2006）02-0109-03 |
| 收稿时间： | 2006-03-19 |
| 修稿时间： | 2006-03-19 |
Cloning and Sequence Analysis of Human T-bet Gene Obtained from Tlymphocyte |
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| YANG Min,WANG Suo-ying,WANG Sheng-jun,MA Jie,MAO Chao-ming,QIU Gu-feng,XU Xiao-peng,SHAO Qi-xiang,XU Hua-xi.Cloning and Sequence Analysis of Human T-bet Gene Obtained from Tlymphocyte[J].Journal of Jiangsu University Medicine Edition,2006,16(2):109-111,119. |
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| Authors: | YANG Min WANG Suo-ying WANG Sheng-jun MA Jie MAO Chao-ming QIU Gu-feng XU Xiao-peng SHAO Qi-xiang XU Hua-xi |
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| Affiliation: | 1. Department of Immunology, School of Medical Technology, Jiangsu University;2. Department of Pediatrics, Affiliated Hospital of Jiangsu University.Zhenjiang Jiangsu 212001, China |
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| Abstract: | Objective: To clone and analyze the cDNA encoding human T-bet expressed in T lymphocyte(T-bet) gene,a type of T-bet family member.Methods: The cDNA of T-bet was amplified from total RNA extracted from human peripheral blood mononuclear cell(PBMC) by RT-PCR and inserted into pGEM-Teasy vector,and the sequence of the DNA was analyzed.Results: The cDNA of T-bet has the length of 1 607 bp with an complete open reading frame,which encodes a product of 530 amino acid and shares 100% homology with the sequence of mRNA for human T-bet in GeneBank.Conclusion: The cDNA of T-bet was successfully cloned,which brought a foundation for further research on its biological function. |
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| Keywords: | RT-PCR |
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