| 苦碟子注射液中腺苷的含量测定 |
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| 引用本文： | 邓意辉,刘丹,吴琼,王绍宁.苦碟子注射液中腺苷的含量测定[J].沈阳药科大学学报,2003,20(6):431-434. |
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| 作者姓名： | 邓意辉 刘丹 吴琼 王绍宁 |
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| 作者单位： | 1. 沈阳药科大学药学院,辽宁沈阳,110016
2. 上海丽珠生物制药有限公司,上海,201206 |
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| 摘 要： | 目的确证苦碟子注射液中腺苷 ,并测定其含量。方法采用HPLC MS法鉴别苦碟子注射液中的腺苷 ;采用HPLC法测定腺苷含量 ,色谱柱为DimonsilTM ODSC18柱 ,流动相为甲醇 乙腈 水(体积比 5∶4∶91 ) ;流速 1mL·min-1;检测波长 2 5 9nm。结果HPLC MS确证了腺苷的存在 ,HPLC测定结果表明腺苷在 0 4～ 3 0 0g·L-1内 ,线形关系良好 (r=0 9999) ,平均回收率为 1 0 0 4% (n=5 )。结论此方法可用于苦碟子注射液中腺苷的含量测定
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| 关 键 词： | 苦碟子注射液 腺苷 液相色谱-质谱联用法 高效液相色谱法 |
| 文章编号： | 1006-2858(2003)06-0431-04 |
| 修稿时间： | 2002年12月19 |
Determination of the content of adenosin in the Ixeris sonchifolia Hance injection |
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| DENG Yi-hui ,LIU Dan ,WU Qiong ,WANG Shao-ning.Determination of the content of adenosin in the Ixeris sonchifolia Hance injection[J].Journal of Shenyang Pharmaceutical University,2003,20(6):431-434. |
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| Authors: | DENG Yi-hui LIU Dan WU Qiong WANG Shao-ning |
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| Affiliation: | DENG Yi-hui 1,LIU Dan 2,WU Qiong 1,WANG Shao-ning 1 |
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| Abstract: | Objective To identify and determine the content of adenosin in the Ixeris sonchifolia Hance injection.Methods The adenosin in Ixeris sonchifolia Hance was identified by HPLC-MS,and determind by HPLC with a Dimonsil TM ODS column( 4.6 mm× 250 mm, 5 μm).The mobile phase consisted of methanol-acetonitrile-water (5∶4∶91,V∶V∶V),the flow rate was 1 mL·min -1.The UV detection wavelength was at 259 nm.Results HPLC-MS proved the presence of adenosin in Ixeris sonchifolia Hance and its injection.In the determination of the content of adenosin by HPLC,the calibration curve was linear (r 2= 0.999 8) within the range of 0.4～ 30.0 mg·L -1,the average recovery was 100.4% (n=5).Conclusion This method is suitable for determination of the content of adenosin in the Ixeris sonchifolia Hance injection. |
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| Keywords: | Ixeris sonchifolia Hance injection adenosin HPLC-MS HPLC |
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