| UV-Vis法测定灵丹草油中总萜类含量 |
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| 引用本文： | 高茗,胡玉霞,余启荣,高柏丽,刘绍兴,和芳.UV-Vis法测定灵丹草油中总萜类含量[J].现代中药研究与实践,2011(1):60-62. |
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| 作者姓名： | 高茗 胡玉霞 余启荣 高柏丽 刘绍兴 和芳 |
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| 作者单位： | 云南盘龙云海药业有限公司;北京创立科创医药技术开发有限公司; |
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| 基金项目： | 国家十一五科技支撑计划项目(项目编号:2006BAI06A18) |
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| 摘 要： | 目的 建立测定灵丹草油中总萜类含量的方法.方法 以β-芹子烯为对照,用5%香草醛-冰乙酸溶液、高氯酸显色,在555 nm波长处测定吸收度.结果 β-芹子烯检测浓度在0.0309～0.13095 mg·mL-1范围内与吸收度呈良好线性关系(r=0.9990),平均回收率为99.38%,RSD=1.23%(n=6).结论 ...
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| 关 键 词： | 紫外-可见分光光度法 灵丹草挥发油 β-芹子烯 含量测定 |
Determination of Total Triterpenoids in Laggera pterodonta(DC.)Benth Oil by UV-Vis Spectrophtometry |
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| GAO Ming,HU Yu-xia,YU Qi-rong,GAO Bai-li,LIU Shao-xing,HE Fang.Determination of Total Triterpenoids in Laggera pterodonta(DC.)Benth Oil by UV-Vis Spectrophtometry[J].Research and Practice on Chinese Medicines,2011(1):60-62. |
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| Authors: | GAO Ming HU Yu-xia YU Qi-rong GAO Bai-li LIU Shao-xing HE Fang |
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| Affiliation: | GAO Ming2,HU Yu-xia1,YU Qi-rong1,GAO Bai-li1,LIU Shao-xing2,HE Fang 1(1.Beijing Supertrack Medical Technology Develop Ltd.,Beijing 100029,China,2.Yunnan PanLongYunHai Pharmaceutical Co.Ltd.,Panlong 675000,China) |
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| Abstract: | Objective To establish UV–Vis Spectrophtometry method for determination of total triterpenoids in Laggera pterodonta（DC.）Benth oil.Methods β-selinene was measured by spectrophotometry with 5% vanillin-glacial acetic acid solution and perchloric acid as colordeveloping agent and detected at 555 nm wavelegth.Results The results showed that it had a good linearity（r=0.999 0）between 0.030 9～0.1309 5 mg·mL-1,the average recovery was 99.38%,with RSD of 1.26%.Conclusion The method was simple,accurate and would be used for the quality control of Laggera pterodonta（DC.）Benth Oil. |
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| Keywords: | UV–Vis Spectrophtometry method Laggera pterodonta（DC ）Benth Oil β-selinene content determination |
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