微生物脂肪酶蛋白质工程*

微生物脂肪酶催化的化学反应具有严格的立体选择性、位点选择性等专一性，催化活性高而副反应少，催化反应不需要辅助因子等特点，因此广泛应用于工农业生产中的诸多领域。利用蛋白质工程技术，提高微生物脂肪酶的特异性、活性和稳定性，对提高微生物脂肪酶制剂产品的市场竞争能力，扩大微生物脂肪酶的应用领域，具有重要的意义。综述了蛋白质工程技术在微生物脂肪酶改性方面的应用现状、存在问题及前景。

Protein Engineering of Microbial Lipases

Microbial lipases are important industrial biocatalysts with the character of stereoselectivity, site selectivity and high catalytic activity with few side effects. They have been used widely in many industrial and agricultural fields. The technology of protein engineering has been successfully applied to improve the activity and stability of microbial lipases, which will raise the competitive capacity of microbial lipase preparations and enlarge theirs application fields.The strategies, the problems and the prospects of protein engineering technology which have been applied to modify the microbial lipases was surveied.