Skp2和p27^kip1蛋白在前列腺癌组织中的表达及临床意义

目的：探讨泛素降解途径蛋白Skp2和p27^kip1在前列腺癌中的表达及与前列腺癌各项临床病理特征的关系,并探讨两者的相关性。方法：应用免疫组织化学法和图像分析系统研究41例前列腺癌标本、20例开放手术切除良性前列腺增生标本中Skp2和p27^kip1的表达情况。分析临床病理资料与免疫组化的结果及Skp2、p27^kip1之间的相关性。结果：在前列腺癌中的Skp2蛋白染色阳性率显著高于良性前列腺增生（P〈0.01）;前列腺癌中的p27^kip1蛋白染色阳性率显著低于良性前列腺增生（P〈0.01）。Skp2蛋白表达与前列腺癌术前前列腺特异性抗原（PSA）水平、肿瘤穿透前列腺被膜、肿瘤分期、病理分级均显著相关（均P〈0.05）。p27^kip1蛋白表达与上述临床病理特征负相关（均P〈0.05）。Skp2和p27^kip1蛋白表达呈负相关（r=-0.572,P〈0.01）。结论：Skp2蛋白在前列腺癌中表达增加,其参与了前列腺癌的发病过程。Skp2蛋白表达与p27^kip1蛋白表达之间负相关。Skp2可能成为前列腺癌治疗的一个新的靶标。

Correlations between Skp2 and p27kip1 protein expressions and clinicopathological features of prostate cancer

Objective:To investigate the correlation between Skp2 and p27kip1 protein expression and the clinicopathological features of prostatic carcinoma,and study the relationships between them.Methods:Skp2 and p27kip1 protein expression were evaluated in the tissues of 41 human prostatic carcinomas as well as 20 benign prostatic hyperplasia(BPH)using immunohistochemistry.Results:The Skp2 labeling frequency in prostatic carcinoma was significantly higher than that in BPH(P<0.01).The p27kip1 labeling frequency in prostatic carcinoma was significantly lower than that in BPH (P<0.01). The Skp2 protein expression in prostatic carcinoma was positively correlated with preoperative serum prostate-specific antigen level,extraprostatic extension,clinical stage and histological grade(P<0.05).The p27kip1 protein expression in prostatic carcinoma were inversely correlated with preoperative serum prostate-specific antigen level,extraprostatic extension,clinical stage and histological grade(P<0.05).Conclusion:Expression of Skp2 protein may lead to decrease in level of p27kip1 in human prostatic carcinoma,indicating its involvement in the development of human prostatic carcinoma.In addition,Skp2 may be a new target for the treatment of prostate cancer.