紫花三叉小白芨组培快繁技术研究

[目的]确定白芨种子萌发的最适培养基,以及种子萌发后在自然光培养下丛生芽诱导分化及生根的最佳条件。[方法]选择成熟白芨果荚为外植体,采用组织培养技术对白芨种子诱导、增殖、生根等问题进行试验研究。[结果]白芨种子诱导最适宜培养基为1/2MB+马铃薯泥20 g/L+蔗糖20 g/L,诱导率为95%;增殖分化培养基为1/2MB+马铃薯泥50 g/L+香蕉泥20 g/L+6-BA0.4 mg/L+NAA 0.1 mg/L+蔗糖25 g/L+艾蒿叶干粉2 g/L,增殖系数为14.2倍;生根培养基为1/2MB+香蕉泥80 g/L+NAA0.5 mg/L+蔗糖20 g/L+艾蒿叶干粉4 g/L,生根率达100%。[结论]白芨组培快繁能培养出带根茎叶的小植株,移栽成活率可达95%,为白芨产业化发展提供技术支持。

Study on Rapid Propagation Technology of Tissue Culture of Purple Flower Trident Small Bletilla striata

[Objective] The research aimed to determine the optimal medium for seed germination of Bletilla striata,the best conditions for inducing differentiation and rooting of multiple shoots under natural light.[Method] The mature Bletilla striata fruit pod was selected as explant,and the tissue culture technique was used to study the inducement,proliferation and rooting of Bletilla striata seed.[Result] The optimum culture medium for Bletilla striata seed induction was 1/2MB + mashed potatoes 20 g/L + sucrose 20 g/L,and the induction rate was 95％.The proliferation differentiation medium was 1/2MB + mashed potatoes 50 g/L + mashed banana 20 g/L +6-BA 0.4 mg/L + NAA 0.1 mg/L +sucrose 25 g/L + Artemisia argyi leaves dry powder 2 g/L,the multiplication coefficient was 14.2.The rooting medium was 1/2MB + mashed banana 80 g/L + NAA 0.5 mg/L + sucrose 20 g/L + Artemisia leaves dry powder 4 g/L,and the rooting rate was 100％.[Conclusion] Rapid propagation of tissue culture of Bletilla striata can cultivate plants with rhizomes and leaves,the survival rate of transplanting can reach 95％,which provides technical support for the industrialization development of Bletilla striata.