The Uptake of Trehalose Glycolipids by Macrophages Is Independent of Mincle |
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| Authors: | Dr Kristel Kodar Selma Eising Dr Ashna A Khan Dr Stefanie Steiger Dr Jacquie L Harper Dr Mattie S M Timmer Dr Bridget L Stocker |
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| Affiliation: | 1. School of Chemical and Physical Sciences, Victoria University of Wellington, P.?O, Box 600, Wellington (New Zealand);2. Malaghan Institute of Medical Research, P.?O, Box 7060, Wellington (New Zealand);3. Centre for Biodiscovery, Victoria University of Wellington, P.?O, Box 7060, Wellington (New Zealand) |
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| Abstract: | Trehalose glycolipids play an important role in the pathogenesis of Mycobacterium tuberculosis and are used as adjuvants for vaccines; however, much still remains unanswered about the mechanisms through which these glycolipids exert their immunomodulatory potential. Recently, the macrophage‐inducible C‐type lectin Mincle was determined to be the receptor for trehalose glycolipids, yet the role played by Mincle in glycolipid uptake is unknown. Accordingly, we developed several fluorescent trehalose glycolipid reporter systems that can be used to study the uptake of soluble trehalose glycolipids and glycolipid‐coated particles by macrophages. Our studies revealed that, although Mincle is essential for the activation of macrophages by trehalose glycolipids, the receptor does not play a role in the uptake of these glycolipids or of glycolipid‐coated particles. |
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| Keywords: | confocal microscopy fluorescent probes macrophages Mincle trehalose glycolipids |
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