生物质谱在核糖核酸领域的应用

综述了近十年来生物质谱用于分析核糖核酸的应用进展。概述了电喷雾电离质谱(ESI-MS)、基质辅助激光解析电离质谱(MALDI-MS)的原理。论述了生物质谱用于分析生物大分子具有准确、快速、灵敏度高等优点。总结了质谱用于单核苷酸多态性分型分析(SNP genotyping);对短的串联重复序列(STR)分析;基因缺陷而导致的疾病诊断;对寡核苷酸片段的序列分析等4个方面的研究成果。讨论了应用ESI-MS分析寡核苷酸形成的非共价复合物,包括研究寡核苷酸的高级结构、寡核苷酸与蛋白质间形成的复合物、寡核苷酸与其它配体小分子间相互作用。提出了存在的问题,并展望了生物质谱未来的发展方向。

The Application Progress on Biological Mass Spectrometry Analysis of Nucleotide

The application progress on biological mass spectrometric analysis of nucleotide has been reviewed in the past decade. The principle of electrospray ionization-mass spec-trometry (ESI-MS) and matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) is described. The advantage of biological mass spectrometry with accuracy, high speed, high sensitivity is introduced. The study results on mass spectrometric analysis of single nucleotide polymorphisms genotyping, short tandem repeats, diagnosis of diseases suffered from gene defect and sequencing of oligonucleotide fragment. The ESI-MS determination of non-covalence complex composed of oligonucleotide is discussed, including study on advanced structure of oligonucleotide, complex of oligonucleotide with protein, interaction between oligonucleotide and other small molecules. Simultaneously, the issue is put forward and the development direction is prospected.