绞股蓝影响白斑癌变过程中Ha-ras基因突变的实验研究

目的 观察绞股蓝对白斑癌变过程中Ｈａ－ｒａｓ癌基因突变的影响，从分子水平探讨绞股莉防癌、抑癌的机理，并对Ｈａ－ｒａｓ基因与白斑癌变动态变化过程的关系进行研究。方法 采用聚合酶链反应－单链构象多态性分析法，检测经绞股蓝总甙、甙元、Ｃ２０－ＯＨ皂甙等处理的的金地鼠颊囊白斑癌变模型标本，观察Ｈａ－ｒａｓ基因含第６１位密码子突变的阳性信号。结果 白斑癌变模型组突变率达２２．９６％，绞股蓝处理平均为７．５９

Experimental study on the influence of Gynostemma pentaphyllam Mak upon point mutation of Ha-ras oncogene in blocking leukoplakia from canceration

Objective To study the influence of Gynostemma pentaphyllam Mak (GP) upon point mutation of Ha ras oncogene the molecular mechanism of GP in blocking canceration ,and the relationship between Ha ras oncogene and canceration of leukoplakia Methods DMBA induced carcinogeneses of the buccal pouch mucosa of the hamsters were divided into leukoplakia model group(LKG)and GP treated group(GPG) Point mutation (codon 61) of Ha ras oncogene was detected by PCR SSCP in both groups Results The mutation incidence of LKG was 22 96% , while the mean incidence of GPG was 7 59% (The incidences of C 20 OH group, glucoside group and aglucon group were 5 32%, 6 25% and 11 00% respectively) When treated with DMBA for 4 to 8 weeks, the mutation incidence of LKG ranged from 11 10% to 40 00%, while GPG ranged from 0 to 10 70% Conclusions GP has outstanding cancer blocking effect chiefly affected by free C 20 OH Mutation of Ha ras oncogence plays an important role in leukoplakia canceration