HPLC法检测抗风湿类中成药中添加的尼美舒利和吡罗昔康

目的：建立HPLC法检测抗风湿类中成药中非法添加化学物质尼美舒利和吡罗昔康的检验方法.方法：采用Diamonsil C18色谱柱,流动相：甲醇-1.36g/L磷酸二氢钾溶液（54∶46）,流速：1.0 ml/min,柱温：25 ℃,检测波长：338 nm.结果：尼美舒利对照品线性范围150.06～400.16 μg/ml,r=0.999 9;吡罗昔康对照品线性范围42.24～112.64 μg/ml,r=0.999 9.平均回收率分别为98.74%,99.04%,RSD分别为0.7%,0.9%（n=6）.结论：本方法灵敏度高,操作简便、准确,可用于定性和定量检测抗风湿类中成药中非法添加的化学物质尼美舒利和吡罗昔康.

Determination of Adding Nimesulide and Piroxicam in Antirheumatic class traditional Chinese medicine by HPLC

Objective：To establish method for determination of Non-steroidal anti-infla mmatory drugs nimesulide and piroxicam , adding illegally in antirheumatic class traditional Chinese medicine by HPLC. Methods：The Diamonsil C18 column was used. The mobile phase was carbinol and potassium dihydrogen phosphate solution （54：46 ）. The flow rate was 1.0 ml/min, and the column temperature was at 25 ℃. The detection wavelength was 338 nm. Results ：The Linear range of reference substance of nimesulide and piroxicam was 150.06 -400.16 μg/ml （r =0. 999 9） and 42.24 - 112.64 μg/ml （r =0.999 9）. The average recovery rate was 98.74% , 99.04% and the relation standard deviation（ RSD ） was 0. 7% ,0. 9% （ n = 6 ）. Conclutions ： The quantitive method is sensitive, simple and accurate, Can be ased for qualitative and quantitative detection of anti-rheumatic class of traditional Chinese medicines illegally added to non-steroidal chemicals nimesulide and piroxicam.