绿原酸防龋的实验研究

目的 研究绿原酸对龋病发生发展的抑制效果。方法 制备不同浓度绿原酸，采用液体稀释法检测其体外抑制变形链球菌和远缘链球菌的效果；建立SD大鼠人工龋模型，分别用绿原酸溶液、甲硝唑洗必泰溶液和蒸馏水给大鼠施药，采用Keyes记分法观察绿原酸对大鼠口腔内龋齿发生发展的抑制效果。结果 绿原酸溶液对变形链球菌ATCC10449株和远缘链球菌OMZ65株的最小抑菌浓度均为2.5 mg/mL，最小杀菌浓度分别为7.5 mg/mL和5 mg/mL。在人工龋大鼠模型中，与蒸馏水相比，绿原酸组龋齿计分明显降低，而与甲硝唑洗必泰组相近。结论 绿原酸对变形链球菌和远缘链球菌生长有明显抑制作用，并可有效抑制大鼠龋齿的发生。

The inhibitory effects of chlorogenic acid on dental caries in rats

Objective To observe the effect of chlorogenic acid on dental caries.Methods The antibacterial activities of chlorogenic acid on Streptococcus mutans ATCC 10449 and Streptococcus sobrinus OMZ65 were investigated.SD rats（n=30） were fed with cariogenic forage 2000# and infected with Streptococcus mutans.The rats were divided into 3 groups,and each group was fed with chlorogenic acid,arilin chlorhexidine solutions or distilled water.The effects of chlorogenic acid on dental caries development in SD rats were measured with Keyes scoring method.Results The MIC of the Streptococcus mutans ATCC 10449 and Streptococcus sobrinus OMZ65 were 2.5 mg/mL.The MBC of the Streptococcus mutans ATCC 10449 and Streptococcus sobrinus OMZ65 were 7.5 mg/mL and 5 mg/mL,respectively.According to the Keyes method,at E,Ds,Dm and Dx level,the scores were lower in chlorogenic acid-treated group when compared with the distilled water control group,but there was no significant difference between the chlorogenic acid-and arilin chlorhexidine solutions-treated groups.Conclusion Chlorogenic acid may inhibit the growth of Streptococcus mutans and Streptococcus sobrinus,and the development of dental caries in SD rats.