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| HPLC-DRC-ICP-MS检测四种尿砷化合物 | |
| 引用本文： | 邓国栋,郑宝山,王学,李社红,王明国,李波,胡军,LU Xiu-fen,X. Chris LE.HPLC-DRC-ICP-MS检测四种尿砷化合物[J].地球与环境,2008,36(3). |
| 作者姓名： | 邓国栋 郑宝山 王学 李社红 王明国 李波 胡军 LU Xiu-fen X. Chris LE |
| 作者单位： | 1. 中国科学院地球化学研究所环境地球化学国家重点实验室,贵州,贵阳,550002;中国科学院研究生院,北京,100039;贵州大学生命科学学院,贵州,贵阳,5500255 2. 中国科学院地球化学研究所环境地球化学国家重点实验室,贵州,贵阳,550002 3. 中国科学院地球化学研究所环境地球化学国家重点实验室,贵州,贵阳,550002;中国科学院研究生院,北京,100039 4. University of Alberta Department of public health Sciences Edmonton Alberta T6G 2G3 Canada |
| 基金项目： | 国家重点基础研究发展计划(973计划)，中国科学院"西部之光"人才培养计划 |
| 摘 要： | 拟建立检测尿液中As(Ⅲ)、DMA(Ⅴ)、MMA(Ⅴ)和As(Ⅴ)4种砷化合物形态的HPLC-DRC-ICP-MS的方法。采用DRCTM(反应气为O2)与HPLC联用作为检测器,Phenomenex ODS-3作为分离柱,5 mmol/L四丁基羟胺,3 mmol/L丙二酸,5%(体积分数)甲醇为流动相(pH=5.0~6.0),建立了同时分析尿液中As(Ⅲ)、DMA(Ⅴ)、MMA(Ⅴ)和As(Ⅴ)的方法。实验结果显示:该分析方法对上述4种砷化合物的检测限均在0.1μg/L以下,在1~20μg/L范围内线性关系良好,准确度和精密度良好,回收率为89%~106%,样品中的Cl-1对测定没有影响。所建立的HPLC-DRC-ICP-MS分析方法稳定、可靠,适用于快速、批量测定尿砷形态。 |
| 关 键 词： | HPLC-DRC-ICP-MS 砷形态 尿液 |
DETERMINATION OF 4 ARSENIC COMPOUNDS IN URINE BY HPLC-DRC-ICP-MS |
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| DENG Guo-dong,ZHENG Bao-shan,WANG Xue,LI She-hong,WANG Ming-guo,LI Bo,HU Jun,LU Xiu-fen,X. Chris LE.DETERMINATION OF 4 ARSENIC COMPOUNDS IN URINE BY HPLC-DRC-ICP-MS[J].Earth and Environment,2008,36(3). | |
| Authors: | DENG Guo-dong ZHENG Bao-shan WANG Xue LI She-hong WANG Ming-guo LI Bo HU Jun LU Xiu-fen X Chris LE |
| Affiliation: | 1.State Key Lab of Environmental Geochemistry;Institute of Geochemistry;Chinese Academy of Sciences;Guiyang 550002;China;2.The Graduate School of the Chinese Academy of Sciences;Beijing 100039;3.College of Life Science;Guizhou University;Guiyang 550025;4.Department of Public Health Sciences;University of Alberta;Edmonton;Alberta;T6G 2G3;Canada |
| Abstract: | The aim of this paper is to establish a method for the simultaneous determination of As(Ⅲ),DMA(Ⅴ),MMA(Ⅴ),and As(Ⅴ) in urine by HPLC-DRC-ICP-MS.The method for the determination of As(Ⅲ),DMA(Ⅴ),MMA(Ⅴ),and As(Ⅴ) in urine was established by using the detector of DRC(O2 as reaction gas) coupled with ICP-MS,and a Phenomenex ODS-3 reverse-phase column with the mobile phase containing 5 mM tetrabutylammonium hydroxide,3 mM malonic acid,and 5%(v/v) methanol(pH5.0～pH6.0).The limits of detection for the four arsenic compounds were below 1μg/L.In the four arsenic compounds,there is a good linearity in the concentration range of 1μg/L～20μg/L with the accepted accuracy and precision.The calculated recoveries were 89 %～106 %.The analysis was not interfered by Cl-in sample matrix.It is concluded that the method has some advantages of high accuracy,fast performance and simple operation,which is suited for rapid and batch determination of arsenic compounds in urine. |
| Keywords: | HPLC-DRC-ICP-MS |
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