生物压电复合陶瓷TCPLNK对大鼠成骨细胞生物相容性的体外研究

目的将生物陶瓷β- 磷酸三钙（β- TCP）与无铅压电陶瓷铌酸锂钠钾（LNK）复合制备具有压电效应的生物材料，研究其与大鼠来源成骨细胞的生物相容性。方法TCP与LNK分别以质量百分比5/5和1/10复合后制备成新型生物压电陶瓷TCPLNK5/5、TCPLNK1/10，极化处理后，标记好正负极。分别在其正负极表面接种大鼠来源的成骨细胞，用扫描电镜、成骨细胞免疫荧光染色观察细胞形态和生长，并用MTT法检测成骨细胞的增殖情况。结果TCPLNK正负极表面接种的成骨细胞早期就表达了良好的黏附性，形态正常，增殖旺盛，呈复层生长。说明了生物压电陶瓷TCPLNK具有优良的生物相容性，其中TCPLNK1/10负极面组成骨细胞的增殖活性高于其他组。结论生物压电复合陶瓷通过模拟天然骨的压电效应可提高骨替代材料的生物相容性。

Biocompatibility of a novel biological piezoelectric ceramic to the rat periosteum derived osteoblast

OBJECTIVE: A novel biological piezoelectric ceramic was made by beta-tricalcium phosphate (beta-TCP) and lithium sodium potassium niobate (LNK) piezoelectric ceramics. To study its biocompatibility to osteoblast isolated from the cranium of 1-day-old Sprague-Dawley mice. METHODS: The biological piezoelectric ceramic TCPLNK1/10, TCPLNK5/5 respectively mixed by beta-TCP and LNK piezoelectric ceramic at the ratio of 1/10 and 5/5. Then osteoblasts were used and seeded respectively on the negative and positive surfaces of TCPLNK1/10 and TCPLNK5/5. Growth and proliferation of the osteoblasts on TCPLNK1/10 and TCPLNK5/5 surfaces were evaluated in vitro by means of scanning electron microscopy (SEM) examination, fluorescence dyeing of osteoblast skeleton protein and MTT assay. RESULTS: Cell morphology of osteoblast on positive and negative surfaces of TCPLNK1/10 and TCPLNK5/5 was normal, and both adhesion and growth characteristics showed better than control group. The growing osteoblasts on the TCPLNK1/10 negative surface were significantly higher than others. The negative surface of TCPLNK1/10 possessed better osteogenesis potential than others in vitro. CONCLUSION: The surface of TCPLNK may permit the imitation piezoelectric effect of natural bone for bone regeneration.