| 高山红景天对肝纤维化大鼠TIMP-1mRNA表达的影响 |
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| 引用本文： | 陈艳军,高旭珍,张运忠,张艳杰,张丽虹.高山红景天对肝纤维化大鼠TIMP-1mRNA表达的影响[J].中华中医药杂志,2010(4). |
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| 作者姓名： | 陈艳军 高旭珍 张运忠 张艳杰 张丽虹 |
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| 作者单位： | 哈尔滨医科大学附属五院;大庆市第二医院门诊部; |
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| 摘 要： | 目的:观察高山红景天对大鼠实验性肝纤维化的治疗效果,并探讨其作用机制。方法:用四氯化碳诱导大鼠肝纤维化模型,将实验动物随机分为正常对照组(A)、模型组(B)、秋水仙碱组(C)、红景天高剂量组(D)、红景天低剂量组(E)。除正常对照组外,其余4组均用四氯化碳诱发肝纤维化,并相应灌药8周。各组于造模第8周末处死动物,分别用放射免疫法检测血清层黏连蛋白(LN),Ⅲ型前胶原(PCⅢ),透明质酸(HA)及Ⅳ型胶原(CⅣ);RT-PCR检测肝组织金属蛋白酶组织抑制因子-1(TIMP-1)mRNA的表达;肝组织HE染色和胶原纤维染色。结果:与模型组大鼠比较,各用药组大鼠血清中LN、HA、PCⅢ、CⅣ水平明显降低(P<0.01);大鼠肝组织内TIMP-1mRNA表达明显下降(P<0.01);大鼠肝组织病理学检测改善显著。结论:高山红景天能有效地抑制肝纤维化的发展,其机制可能是通过下调肝组织内TIMP-1mRNA表达,降低ECM的分泌而达到的。
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| 关 键 词： | 肝纤维化 高山红景天 金属蛋白酶组织抑制因子-1 细胞外基质（ECM） |
Effects of Sachalin rhodiola rhizome on TIMP-1 mRNA expression of rat with hepatic fibrosis |
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| CHEN Yan-jun,GAO Xu-zhen,ZHANG Yun-zhong,ZHANG Yan-jie,ZHANG Li-hong.Effects of Sachalin rhodiola rhizome on TIMP-1 mRNA expression of rat with hepatic fibrosis[J].China Journal of Traditional Chinese Medicine and Pharmacy,2010(4). |
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| Authors: | CHEN Yan-jun GAO Xu-zhen ZHANG Yun-zhong ZHANG Yan-jie ZHANG Li-hong |
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| Affiliation: | 1Department of Digesive Diseases;the Fifth Affiliated Hospital of Harbin Medical University;Daqing 163316;China;2Out-Patient Clinic;the Second Hospital of Daqing City;Daqing 163461;China |
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| Abstract: | Objective: To investigate the therapeutic effects and mechanism of Sachalin rhodiola rhizome on TIMP-1mRNA expression in hepatic fibrosis rats. Methods: Hepatic fibrosis rat models were established with carbon tetrachloride (CCl4). All the experimental rats were divided into five groups: normal group (A), model group (B), colchicines group(C), high dosage of Sachalin rhodiola rhizome (D) and low dosage of Sachalin rhodiola rhizome (E). All groups except A were given carbon tetrachloride to induce the rat mo... |
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| Keywords: | Sachalin rhodiola rhizome Hepatic fibrosis Tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) Extracellular matrix (ECM) |
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