居间驹形氏杆菌发酵大豆糖蜜生产细菌纤维素条件的优化

通过居间驹形氏杆菌(Komagataeibacter intermedius CGMCC12562)发酵生产细菌纤维素,以大豆糖蜜作主要发酵原料,优化发酵工艺参数,提高细菌纤维素产量。在单因素试验的基础上,筛选出对细菌纤维素产量影响较大的4个因素,即大豆糖蜜可溶性固形物含量、玉米蛋白粉、ZnSO_4及苹果酸添加量,并通过Design-Expert响应面分析对这4个因素进行优化,得到最优的培养基及发酵工艺为:大豆糖蜜可溶性固形物含量14.13°Brix、玉米蛋白粉添加量1.6%、ZnSO_4添加量0.11%、苹果酸添加量0.41%;在30℃、接种量10%、装料量16%、初始pH 6.0的条件下静置培养7d,细菌纤维素产量高达(15.68±0.82)g/L。

Optimization of Bacterial Cellulose Production by Fermented Soybean Molasses with Komagataeibacter intermedius

In this study, Komagataeibacter intermedius CGMCC12562 was employed to produce bacterial cellulose (BC) from soybean molasses. The fermentation process was optimized to improve BC yield. Through one-factor-at-a-time experiments, the soluble solids content (SSC) of soybean molasses, and corn gluten meal, ZnSO4 and malic acid were selected as the factors with a significant influence on BC production for optimization by response surface methodology. It was found that the optimized culture medium consisted of soybean molasses with an SSC level of 14.13 °Brix, 1.6% corn gluten meal, 0.11% ZnSO4 and 0.41% malic acid. The BC yield was as high as (15.68 ± 0.82) g/L after 7 days of culture at 30 ℃ for in a 250-mL conical flask containing 16% of the medium with an inoculum size of 10% at an initial pH of 6.0 under static?cultivation?conditions.