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| TWEAK蛋白对肝癌细胞增殖的影响 | |
| 引用本文： | 程晓刚,陈玮,陈恬,李晋川,邹强,杨淑霞,刘阳,李敏惠,白云.TWEAK蛋白对肝癌细胞增殖的影响[J].免疫学杂志,2007,23(5):510-513. |
| 作者姓名： | 程晓刚 陈玮 陈恬 李晋川 邹强 杨淑霞 刘阳 李敏惠 白云 |
| 作者单位： | 1. 成都医学院,病原学教研室,成都,610083 2. 成都医学院,科研中心,成都,610083 3. 第三军医大学分子遗传学教研室,重庆,400038 |
| 摘 要： | 目的 观察TWEAK蛋白对肝癌细胞系增殖的影响,探讨TWEAK对肝癌细胞增殖的作用.方法构建PAVU6-TWEAK siRNA干涉质粒,转染HepG2和QGY7703肝癌细胞株,MTT法检测抑制TWEAK蛋白表达对肝癌细胞增殖的影响,并于培养体系中加入TWEAK蛋白,检测肝癌细胞增殖活性.结果成功构建PAVU6-TWEAK siRNA干涉质粒,转化肝癌细胞后肝癌细胞增殖受抑.肝癌培养体系中加入TWEAK蛋白后,肝癌细胞增殖活性有所增强.结论 TWEAK蛋白可促进肝癌细胞的增殖活性,抑制TWEAK表达,一定程度上可抑制肝癌细胞增殖. |
| 关 键 词： | TWEAK 肝癌 RNA干涉 增殖 TWEAK 蛋白表达 肝癌细胞增殖 影响 cell proliferation human hepatoma 程度 细胞增殖活性 增强 转化 结果 培养体系 检测 肝癌细胞株 转染 干涉 siRNA 方法 作用 肝癌细胞系 |
| 文章编号： | 1000-8861（2007）05-0510-04 |
| 修稿时间： | 2006-11-212007-03-19 |
Effects of TWEAK on human hepatoma cell proliferation |
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| CHENG Xiao-gang,CHEN Wei,CHEN Tian,LI jin-chuan,ZOU Qiang,YANG Shu-xia,LIU Yang,LI Min-hui,BAI Yun.Effects of TWEAK on human hepatoma cell proliferation[J].Immunological Journal,2007,23(5):510-513. | |
| Authors: | CHENG Xiao-gang CHEN Wei CHEN Tian LI jin-chuan ZOU Qiang YANG Shu-xia LIU Yang LI Min-hui BAI Yun |
| Affiliation: | Department of Pathogeny, Chengdu Medical College, Chengdu 610083, China |
| Abstract: | Objective To investigate the effectS of TWEAK on human hepatoma cell proliferation. Methods The PAVU6-TWEAK siRNA interference vectors were constructed and transfected into hepatoma cell line and the TWEAK protein was added into cell culture to evaluate the effects of inhibiting TWEAK expression on hepatoma cells proliferation using MrIT methods. Results After transfection, the proliferation of hepatoma cell line was inhibited. On the contrary, the proliferation of hepatoma cells was increased by adding TWEAK protein into cell culture. Conclusion The TWEAK expression levels have some influence upon hepatoma cell proliferation. Inhibition of TWEAK expression could decrease human hepatoma cell proliferation. |
| Keywords: | TWEAK Hepatoma cells RNA Interference Proliferation |
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