建立H5N1流感病毒感染雪貂动物模型

目的建立甲型H5N1流感病毒感染雪貂动物模型1-3]。方法 H5N1流感病毒株A/Vietnam/1203/2004病毒以103和104 TCID50滴度分别感染雪貂。对4～10月龄去势雪貂经兽用氯胺酮轻度麻醉后进行滴鼻感染,每个稀释度接种3只雪貂,感染后第5天安乐处死。感染后每天记录雪貂一般临床变化。感染前0 d采集鼻甲骨活检,感染后1～5 d鼻甲骨活检检测病毒载量和病毒滴度。处死时取雪貂气管、肺、心、肝、脾、肾、小肠、脑组织作病毒滴度检测和病理检查。结果 H5N1103和104 TCID50的病毒分别感染雪貂,雪貂死亡都率在33%。103TCID50和104 TCID50病毒分别感染雪貂,动物都出现持续3 d体温升高,104 TCID50组出现超过20%的体重下降。上呼吸道排毒呈现上升趋势,并可在除呼吸系统以外的组织器官中分离到病毒。感染的雪貂病理表现为重度肺炎。结论雪貂感染H5N1病毒株后在临床表现、病毒学、分子生物学、病理学方面的检测都可以证实雪貂感染H5N1病毒动物模型已建立,104 TCID50病毒滴度是一个建立感染动物模型比较合适的剂量。

Establish H5N1 Flu Virus Infection Ferret Animal Model

Objective To establish the Influenza H5N1 influenza virus infection ferrets animal model.Methods Groups of three 4-to 6-month-old ferrets were inoculated intranasally with 103 and 104TCID50 of H5N1 A /Vietnam/1203/2004 in a volume of 500uL.Ferrets were monitored daily for clinical signs of influenza infection,and body temperatures were recorded once daily.Every day take nasal turbinates for RT-PCR.On day 5 postinoculation,ferrets were euthanized and trachea,lung,heart,liver,slpeen,kidney,small intestine,and...