5-氮杂-2＇-脱氧胞苷对人肺腺癌A549细胞株的生长抑制作用

目的：探讨5-氮杂-2＇-脱氧胞苷（5-Aza-dC）对人肺腺癌A549细胞株的抑制作用及其机制。方法：采用四甲基偶氮噻唑氮蓝（MTT）法测定5-Aza-dC对A549细胞生长的影响;考马斯亮蓝G-250染色法测定5-Aza-dC作用后A549细胞蛋白质含量;Hoechst 33258荧光染色检测A549细胞凋亡和形态改变。结果：40～100μg/mL 5-Aza-dC对A549细胞的增殖有明显抑制作用,与对照组比较差异均有统计学意义（P〈0.05,P〈0.01）,并具有明显的时效和量效关系,同时蛋白质相对含量下降。60～100μg/mL 5-Aza-dC作用后A549细胞出现明显形态改变且凋亡增加。结论：5-Aza-dC对人肺腺癌A549细胞的生长具有抑制作用,并引起蛋白质相对含量明显下降和细胞凋亡,其机制可能与阻滞细胞周期,蛋白质功能改变及诱导细胞凋亡有关。

Growth inhibition of 5-Aza-2'-deoxycytidine on human lung adenocarcinoma cell line A549

Objective： To investigate the inhibition of 5-Aza-2＇ deoxycytidine（5-Aza-dC） on human lung adenocarcinoma cell line A549 and its possible mechanism.Methods： Use MTT method to find the differences of the effect on A549 cell growth;use Coomassie Brilliant Blue G-250 staining to determine the relative protein content of the tested A549 cell;use Hoechst 33258 fluorescence staining to observe the apoptosis of A549 cell and its morphological variations.Results： When the 5-Aza-dC was given in a certain range of concentration（40-100 μg / mL）,the A549 cell proliferation could be significantly inhibited in a dose and time manner,compared with the control group（P 0.05,P 0.01）.Meanwhile,the relative content of protein in the tested A549 cell was significantly decreased.When the concentration was in the range of 60-100 μg / mL,the apoptosis of A549 cell and its morphological variations could be observed.Conclusion： 5-Aza-dC can inhibit the growth of human lung adenocarcinoma A549 cells,induce the apoptosis and decrease the relative content of the protein.Its mechanism can be related to the block of cell cycle,alternation of the protein function and apoptosis induction.