瘦素减轻鱼藤酮诱导的SH-SY5Y细胞损伤

目的 探讨瘦素对鱼藤酮（rotenone） 所诱导的人神经母细胞瘤SH-SY5Y 细胞凋亡的抑制作用。 方法 建立鱼藤酮诱导的SH-SY5Y 细胞损伤模型，用瘦素进行干预，采用MTT 比色法检测细胞存活率，锥虫蓝检测细胞死亡率，细胞形态学观察，并用Western blot 法检测Bcl-2、Bax、p-GSK-3β（Ser9） 和GSK-3β 的表达水平，免疫荧光检测α-synuclein 的表达水平。 结果 成功建立了鱼藤酮诱导的SH-SY5Y 细胞损伤模型。在模型的基础上，瘦素干预后，细胞状态改善，细胞存活率提高约14%（P ＜ 0.05），细胞死亡率降低约17%（P ＜ 0.05），α-synuclein 的浓度下降，Bcl-2 和p-GSK-3β 的表达显著提高。 结论 瘦素对鱼藤酮诱导的SH-SY5Y 细胞损伤具有明显的抑制作用，可能是通过抑制GSK-3β 的活性上调Bcl-2/Bax 的比率和降低α-synuclein 的表达而得以实现。

Leptin alleviates rotenone-induced injury of SH-SY5Y cells

Objective To study the inhibitory effect of leptin on rotenone-induced apoptosis of human neuroblastoma SH-SY5Y cells. Methods A SH-SY5Y cell injury model was induced by rotenone and intervened with leptin. Viability of SH-SY5Y cells was assayed by MTT, apoptosis of SH-SY5Y cells was detected with trypan blue staining, morphology of SH-SY5Y cells was observed under inverted microscope, expression levels of Bcl-2, Bax, p-GSK-3 β （Ser9）, GSK-3 β and α -synuclein were measured by Western blot and immunofluorescence, respectively. Results The rotenone-induced SH-SY5Y cell injury model was successfully established. The survival rate of SH-SY5Y cells was about 14% higher（P 〈 0.05）, the apoptosis rate of SH-SY5Y cells was about 17% lower（P 〈 0.05）, the α -synuclein expression level was significantly lower whereas Bcl-2 and p-GSK-3 β expression was significantly higher after leptin intervention than before leptin intervention. Conclusion Leptin can significantly inhibit rotenoneinduced SH-SY5Y cell injury by up-regulating the Bcl-2/Bax ratio and down-regulating the expression of α -synuclein through the activity of GSK-3 β .