喉鳞癌组织NF—kB的表达和淋巴管计数的临床意义及相关性研究

目的研究喉鳞癌组织中核因子-kB（NF—kB）表达水平和淋巴管计数，探讨两者在喉鳞癌中的临床病理意义及相互关系。方法收集50例喉鳞癌手术切除组织标本，另取10例声带息肉组织作对照，常规制作石蜡包埋切片，NF-kB表达的检测和淋巴管计数均采用SP免疫组化法。结果喉鳞癌NF-KB表达阳性率和淋巴管计数为明显高于声带息肉组60％vs10％，P〈0．05，（13．3±3．4）个／HP vs（6．1±3．8）个／HP，P〈0．01]；高分化喉鳞癌和未转移病例NF—KB表达阳性率和淋巴管计数明显低于低分化喉鳞癌和转移病例33．3％vs84．2％，（9．6±2．7）个／HP vs（16．9±3．4）个／HP，P〈0．05或P〈0．01]；NF—KB表达阳性的喉鳞癌淋巴管计数明显高于阴性表达者（14．9±4．1）个／HP vs（9．8±3．1）个／HP，P〈0．01]。结论NF—kB表达和淋巴管计数均为反映喉鳞癌进展、转移等生物学特性及预后的重要标记物，喉鳞癌细胞分泌的NF—kB可能促进淋巴管生成。

The clinical study of nuclear factor-kappa B expression and counts of lymph vessels in laryngeal squamous cell carcinoma and polyps of vocal cord tissues

Objective To study the expression of nuclear factor-kappaB （NF-kB） and the counts of lymph vessels in laryngeal squamous cell carcinoma and polyps of vocal cord tissues, and explore their clinicopathologic significance and correlation in the course of laryngeal carcinoma. Methods SP immunohistochemical method was used to detect the expression of NF-kB and the counts of lymph vessels on the routinely paraffin-embedded sections of the specimens from 50 cases laryngeal squamous cell carcinoma and 10 cases of polyps of vocal cord tissues. Results The positive rate of NF-KB and the counts of lymph vessels in laryngeal carcinoma 60. 0%, （13.3 ± 3.4 ）/HP] were significantly higher （ P 〈 0. 05 and P 〈 0. 01 respectively） than those in polyps of vocal cord tissues 10. 0 % , （6. 1 ± 3. 8）/HP]. The positive rate of NF-kB and the counts of lymph vessels in well differentiated adenocarcinoma and cases without metastasis were significantly lower（ P 〈 0. 05, P 〈 0. 01 ）, compared with poor-differentiated adenocarcinoma and cases with metastasis. The counts of lymph vessels in the NF-kB positive cases were significantly higher than those in NF-KB negative cases （ 14. 9± 4. 1 ） / HP vs （9. 8±3.1 ） / HP, P 〈 0.01 ]. Conclusions The expression of NF-KB and the counts of lymph vessels might be important markers to be used to monitor the progression, biological behaviors, metastatic status and prognosis of laryngeal carcinoma. NF-kB might promote lympoangiogenesis in laryngeal squamous cell carcinoma tissues.