| 应用光敏生物素标记核酸探针鉴定分枝杆菌的研究 |
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| 引用本文： | 田润芝, 孙茹芹, 王玖芬, 王玉铭, 王丽华, 韩珍,.应用光敏生物素标记核酸探针鉴定分枝杆菌的研究[J].微生物学通报,1995,22(5):293-297,319. |
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| 作者姓名： | 田润芝 孙茹芹 王玖芬 王玉铭 王丽华 韩珍 |
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| 作者单位： | 哈尔滨市结核病防治所; |
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| 摘 要： | 用光敏生物素标记非结核分枝杆菌临床分离株及标准分枝杆菌全染色体ＤＮＡ制成探针，与已知标准牛分枝杆菌（ＢＣＧ株）及不同种的非结核分枝杆菌ＤＮＡ杂交，可快速将分支杆菌鉴定到种，同时以大肠埃希氏菌做阴性对照，显示良好的特异性和准确性。此种方法具有鉴定速度快、操作简便、稳定性好及对人体无害等特点，适用于结核杆菌和非结核分枝杆菌的菌种鉴定。
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| 关 键 词： | 分枝杆菌 光敏生物素标记核酸探针 DNA－DNA杂交 鉴定 |
THE IDENTIFICATION OF MYCOBACTERIA BY PHOTOBIOTINMARK NUCLEI ACID PROBES |
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| Tian Runzhi,Sun Ruqin,Wang Jiufen,Wang Yuming,Wang Lihua ,Han Zhen.THE IDENTIFICATION OF MYCOBACTERIA BY PHOTOBIOTINMARK NUCLEI ACID PROBES[J].Microbiology,1995,22(5):293-297,319. |
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| Authors: | Tian Runzhi Sun Ruqin Wang Jiufen Wang Yuming Wang Lihua Han Zhen |
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| Abstract: | The DNA probes from separation strain of non-tuberculosis mycobacteria,and panchromosome of standard mycobaclerium spp. labelled by photo-sensitive biotin werehybridized with DNAs from known standard mycobacterium bovis (BCG strain)and variousnon-tuberculosis mycobacteria to identify mycobacteria strains by negative contrasl withEscherichia coli into species levcl rapidly with good specificity and accuracy. The chara-cteristics of this method include short-time consumed identification, ease operation, goodstability and noharm to human, and it is suitable to the identification of Mycobacleriumtubcrculosis and non-tuberculosis mycobacteria. |
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| Keywords: | Mycobacterium Photobiotin mark nuclei acid probes DNA DNA Hyridi-zation Identification |
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