解离素-金属蛋白酶15与慢性心房颤动心房结构重构的关系

目的探讨解离素-金属蛋白酶15(ADAM15)与慢性心房颤动(房颤)心房结构重构的关系。方法选择30例心外科手术患者,按有无房颤病史分为房颤组(n=15)和无房颤组(n=15)。取患者右心耳心肌组织后经Masson染色测定组织胶原容积分数(CVF)、RT-PCR法检测ADAM15基因mRNA的表达、Western blot法检测ADAM15蛋白的表达。结果与无房颤组比较,房颤组左心房直径增大(41.5±10.4)mmvs(49.6±5.0)mm,P0.05]、CVF明显升高(11.67±3.35vs42.38±9.79,P0.01)、ADAM15mRNA(0.51±0.04vs1.20±0.06,P0.01)及蛋白表达(1.19±0.05vs2.61±0.16,P0.01)上调。ADAM15蛋白与左房直径(r=0.88,P0.01)、房颤持续时间(r=0.83,P0.01)及CVF(r=0.78,P0.05)呈正相关。结论AD-AM15可能参与心房颤动时心房的结构重构。

Relationship between a disintegrin-metalloproteinase15 and atrial structural remodeling of chronic atrial fibrillation

Objective To investigate the relationship between a disintegrin-metalloproteinase15 （ADAM15） and atrial structural remodeling of chronic atrial fibrillation （Af）.Methods The tissue of right atrial appendage were collected from 30 patients with cardiac surgery,and then divided into Af group （n=15） and non-Af group （n=15） according to whether the patients had Af history or not.The atrial collagen volume fraction （CVF） was determined after Masson staining,the gene expression and the protein expression of ASAM15 were detected by RT-PCR and Western blotting respectively in two groups.Results Compared with the non-Af group,in Af group the left atrial diameter increased （4.15±1.04 vs 4.96±0.50,P0.05）,CVF increased significantly （11.67±3.35 vs 42.38±9.79,P0.01）,the expression of ADAM15-mRNA was up-regulated （0.51±0.04 vs 1.20±0.06,P0.01）,and protein expression of ADAM15 was up-regulated too （1.19±0.05 vs 2.61±0.16,P0.01）.ASAM15 protein was correlated to the left atrial diameter （r=0.88,P0.01）,Af duration （r=0.83,P0.01） and CVF （r=0.78,P0.05）.Conclusion ADAM15 may play a role in the remodeling of atrial structure during Af.