| 性激素对心钠素基因表达影响的实验研究 |
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| 引用本文： | 李志盛,殷蓉蓉,洪敏,刘剑凯,李小林.性激素对心钠素基因表达影响的实验研究[J].吉林大学学报(医学版),1992(3). |
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| 作者姓名： | 李志盛 殷蓉蓉 洪敏 刘剑凯 李小林 |
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| 作者单位： | 白求恩医科大学分子生物学中心实验室，通化卫生学校，白求恩医科大学生物化学教研室，白求恩医科大学生物化学教研室，白求恩医科大学生物化学教研室 |
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| 摘 要： | 为研究雄激素和雌激素对心钠素基因表达的影响,给完整的、去势的雄性大鼠皮下注射丙酸睾丸酮或雌二醇,持续2周。用冷酚法提取心房总RNA,用α-~(32)P标记ANF—cD-NA探针,经RNA电泳转移杂交和斑点吸印杂交。结果表明去势后ANF—mRNA含量下降,睾丸酮和雌二醇均使ANF—mRNA增加,然而小剂量的睾丸酮、雌二醇对去势大鼠ANF基因表达作用还需进一步研究。
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| 关 键 词： | 心钠素 基因表达 雄激素 雌激素 |
THE EFFECTS OF SEX HORMONE ON ATRIAL NATRIURETIC FACTOR (ANF) GENE EXPRESSION IN VIVO |
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| Li Zhisheng,Hong Min,Yin Rongrong,Liu Jiankai,Li Xiaolin.THE EFFECTS OF SEX HORMONE ON ATRIAL NATRIURETIC FACTOR (ANF) GENE EXPRESSION IN VIVO[J].Journal of Jilin University: Med Ed,1992(3). |
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| Authors: | Li Zhisheng Hong Min Yin Rongrong Liu Jiankai Li Xiaolin |
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| Abstract: | In order to clarify the effects of androgen and estrogen on the expression of ANF gene in vivo, the intact and emasculated male rats were exposed to testosterone or estradiol by subcutaneous injection for 2 weeks, once a day. The atrial total RNA was extracted by cold phenol method and was hybridized with a-32P labeled ANF -cDNA probe. The results revealed that the ANP - mRNA level of emasculated rats was lower than that of the intact rats: the large dose of testosterone increased the ANF -mRNA level in castrated rats, so did in the intact ones. Estradiol also enhanced the ANF-mRNA level in castrated rats. Whereas, the effects of small dose of testosterone and estradiol on ANF gene expression were needed to be studied further. |
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| Keywords: | ANF Gene expression Androgen Estrogen |
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