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Influence of the pressure shift freezing and thawing on the microstructure of largemouth bass
Affiliation:1. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China;2. Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture, Ministry of Agriculture, Hangzhou 310058, China;3. Department of Food Science and Agricultural Chemistry, McGill University, 21111 Lakeshore Road, St-Anne-de-Bellevue, QC H9X 3V9, Canada;1. Universidad Politécnica de Madrid, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Departamento de Ingeniería Agroforestal, Spain;2. Universidad Politécnica de Madrid, Escuela Técnica Superior de Edificación, TEMA Research Group, Spain;1. Department of Food Science and Technology, University of California, Davis, CA, USA;2. Department of Nutrition and Food Science, University of Maryland, College Park, MD, USA;3. Department of Biological and Agricultural Engineering, University of California, Davis, CA, USA;1. Research Laboratory of Microbiology (RLM), Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadat Campus, Beirut, Lebanon;2. Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadat Campus, Beirut, Lebanon;3. Centre d''Analyses et de Recherche (CAR), Unité de Recherche Technologies et Valorisation agro-Alimentaire (UR-TVA), Faculté des Sciences, Université Saint-Joseph de Beyrouth, Campus des sciences et technologies, Mar Roukos, Matn, Lebanon;1. Department of Food Engineering, Ankara University, Gölbasi-Ankara, Turkey;2. Department of Food Engineering, Gümüshane University, Gümüshane, Turkey;3. Department of Food Engineering, Middle East Technical University, Ankara, Turkey;1. Glyn O. Phillips Hydrocolloid Research Centre at HBUT, National “111” Center for Cellular Regulation and Molecular Pharmaceutics, School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, China;2. Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK;3. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada
Abstract:The objective of this study was to use a new self-cooling laboratory system for carrying out the pressure shift freezing (PSF) and evaluate the influence of PSF at 150 MPa on the microstructural properties of largemouth bass relative to liquid immersion freezing (LIF) and conventional air freezing (CAF). CAF, LIF and PSF showed average total freezing times of 176 ± 7.4, 65.3 ± 6.8 and 23.2 ± 3.1 min, and the cross sectional area of ice crystals in the muscle were 1002 ± 778, 501 ± 248 and 143 ± 50.6 μm2, respectively, demonstrating a significant reduction in crystal size to be associated with PSF. It was observed that damage caused by the ice crystals during the freezing to the muscle microstructure was irreversible. The thawing and cooking losses of largemouth bass after the freezing were lower for PSF as compared to the other two freezing methods. PSF reduced the damage to myocytes and resulted in lower drip loss due to reduced microstructure disruption due to their small ice crystals, thereby maintaining the muscle tissue to better retain the fluids. Color and texture properties were less affected by PSF.Industrial relevanceFreezing is the most used preservation method for aquatic products. Rapid freezing results in better texture retention while the slow freezing damages the product texture because of the formation of extracellular large ice crystals developed during the freezing process. Thus, the nature of freezing affects the quality of frozen foods. Successful freezing processes aim at employing rapid freezing conditions which result in the formation of small ice crystals. Pressure shift freezing (PSF) is a novel technique with advantages of high degree of super-cooling, short phase transformation time, and results in very small ice crystals. This study makes use of a laboratory self-cooling system to carryout PSF of largemouth bass. This cooling system overcomes the limitation of previous studies on PSF which are expensive, limited to small size and impractical for commercial exploitations. The cooling system employed in this study can be easily adapted to large-scale production of PSF aquatic products. Test results provide a basis for the commercial exploitation of PSF for largemouth bass and such other aquatic foods for driving the quality advantage.
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