Monocyte exposure to fine particulate matter results in miRNA release: A link between air pollution and potential clinical complication |
| |
| Affiliation: | 1. Jagiellonian University Medical College, Coronary Disease Department and Heart Failure, John Paul II Hospital, Kraków, Poland;2. Jagiellonian University Medical College, Faculty of Health Sciences, Department of Emergency Medicine, Kraków, Poland;3. Jagiellonian University Medical College, 2nd Department of Internal Medicine, Kraków, Poland;4. Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland;5. Jagiellonian University Medical College, Faculty of Medicine, Department of Bioinformatics and Telemedicine, Kraków, Poland;6. AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Kraków, Poland;7. Max Planck Institute for Biogeochemistry in Jena, Hans-Knöll Jena, Germany;8. Department of Biogeochemical Signals, Max Planck Institute for Biogeochemistry, Hans-Knöll Str. 10, 07745 Jena, Germany;9. AGH University of Science and Technology, Faculty of Energy and Fuels, Kraków, Poland;1. Departamento de Genética, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay;2. Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay;3. Laboratorio de Microbiología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay;1. Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China;2. Faculty of Public Health, Pyongyang Medical University, Pyongyang, Democratic People''s Republic of Korea;1. Division of Pulmonary and Critical Care Medicine, Department of Medicine, Pennsylvania State University, College of Medicine, Hershey, PA, USA;2. Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA, USA;3. Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of California, Irvine, CA, USA;4. Department of Emergency Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA;5. Rocky Mountain Poison and Drug Center, Denver Health and Hospital Authority, Denver, CO, USA;6. Department of Medicine, University of California, San Diego, La Jolla, CA, USA;7. Heart and Vascular Institute, Department of Medicine, Pennsylvania State University, College of Medicine, Hershey, PA, USA;1. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok Thailand;2. Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok Thailand;1. Department of Occupational Health and Occupational Diseases, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China;2. The Key Laboratory of Nanomedicine and Health Inspection of Zhengzhou, Zhengzhou 450001, Henan, China;3. Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, Henan, China;1. Bioscience Research Center, 58015 Orbetello, GR, Italy;2. Dipartimento di Scienze della Vita, Università degli Studi di Trieste, 34127 Trieste, Italy;3. National Research Council—Institute for Biological Resources and Marine Biotechnologies (IRBIM), Lesina, Italy;4. Catalan Institute for Water Research (ICRA-CERCA), 17003 Girona, Spain;5. Institute of Environmental Assessment and Water Research (IDAEA-CSIC), 08034 Barcelona, Spain;6. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d′Aosta, 10154 Torino, Italy |
| |
| Abstract: | Chronic exposure to PM2.5 contributes to the pathogenesis of numerous disorders, although the underlying mechanisms remain unknown. The study investigated whether exposure of human monocytes to PM2.5 is associated with alterations in miRNAs. Monocytes were exposed in vitro to PM2.5 collected during winter and summer, followed by miRNA isolation from monocytes. Additionally, in 140 persons chronically exposed to air pollution, some miRNA patterns were isolated from serum seasonally. Between-season differences in chemical PM2.5 composition were observed. Some miRNAs were expressed both in monocytes and in human serum.MiR-34c-5p and miR-223-5p expression was more pronounced in winter. Bioinformatics analyses showed that selected miRNAs were involved in the regulation of several pathways. The expression of the same miRNA species in monocytes and serum suggests that these cells are involved in the production of miRNAs implicated in the development of disorders mediated by inflammation, oxidative stress, proliferation, and apoptosis after exposure to PM2.5. |
| |
| Keywords: | Fine particulate matter Monocytes MiRNA expression Civilization disorders |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
