祁术的组织培养及快速繁殖技术研究

为研究祁术的组织培养及快速繁殖技术,选用祁术的叶片和下胚轴为外植体材料,接种于附加植物生长调节剂NAA,6-BA及其组合的MS固体培养基上.结果表明:下胚轴为外植体明显好于叶片,对于愈伤组织形成,叶片采用MS NAA(0.5mg/L) 6-BA(2mg/L);下胚轴MS NAA(0.5mg/L) 6-BA(2mg/L);MS NAA(0.5mg/L) 6-BA(1mg/L)为宜.对于叶片丛生芽愈伤组织培养基用MS NAA(0.2mg/L) 6-BA(2.8mg/L),下胚轴丛生芽愈伤组织培养基用MS NAA(0.01mg/L) 6-BA(0.1mg/L);MS NAA(0.05mg/L) 6-BA(0.1mg/L)为宜,诱导生根以1/2MS NAA0.3mg/L最佳,生根率达95%以上.

Study on Atractylodes macroephala Koidz By Tissue Culture and Rapid Propagation

For the study on the rapid propagation of Atractylodes macroephala Koidz,the aseptic inferior embryo spindles and leaves were used as explants cultured in solid medium MS supplemented with NAA,6-BA or combinations of plant growth regulators with different concentrations .The results showed that Better results could be obtained by the inferior Embryo spindle; the optimum combination was MS NAA(0.5mg/L) 6-BA(2mg/L) for the leaves, MS NAA(0.5mg/L) 6-BA(2mg/L) for the inferior embryo spindle; for bud increasing, the leaf is MS NAA(0.2mg/L) 6-BA(2.8mg/L),and the inferior embryo spindle is MS NAA(0.01mg/L) 6-BA(0.1mg/L);for the increasing of root, we should use 1/2 MS NAA(0.5mg/L) ,and the effect can reach more than 95%.