内皮素1对人A375黑素瘤细胞株细胞黏附及细胞黏附分子1表达的影响

目的 探讨内皮素（ET）－1对人A375黑素瘤细胞增殖、黏附、迁移及细胞黏附分子（ICAM）－1表达的影响。方法 MTT法观察ET－1对人A375黑素瘤细胞生长的影响，黏附实验检测细胞黏附，Transwell迁移系统检测细胞迁移，流式细胞仪检测对人A375黑素瘤细胞ICAM－1表达的影响。结果 ET-1在0.002 ～ 0.2 μg/mL浓度范围可促进黑素瘤细胞的增殖、在纤连蛋白上的黏附、通过微孔滤膜及抑制ICAM－1的表达（P < 0.01），在0.2 μg/mL时作用最强，细胞代谢活性（24 h）、细胞黏附率、ICAM-1含量（24 h）分别为0.327 ± 0.009、163.31 ± 4.05、4.667 ± 0.551；在0.2 ～ 2 μg/mL浓度范围作用相反。结论 ET-1可能通过抑制ICAM-1的表达、促进细胞增殖、黏附及迁移而增强细胞代谢、增加色素形成。

Effects of endothelin-1 on the adhesion and expression of intercellular adhesion molecule 1 by A375 human malignant melanoma cells

Objective To observe the effect of endothelin-1 (ET-1) on the cell growth, adhesion, migration and expression of intercellular adhesion molecule 1 (ICAM-1) by A375 human malignant melanoma cells. Methods A375 cells were cultured in the presence of ET-1 of various concentrations (0.002, 0.02, 0.2, 2 μg/mL) for different periods. MTT method and flow cytometry were applied to detect the proliferation and ICAM-1 expression of these cells, respectively, after 24-, 48-, and 72-hour treatment. After 24-hour treatment, the cell adhesion and migration of A375 cells were assessed with cell adhesion assay and Transwell chambers, respectively. Results In the case of ET-1 from 0.002 to 0.2 μg/mL, it enhanced the proliferation, adhesion, migration of A375 cells and inhibited the expression of ICAM-1 by A375 cells in a dose dependent manner (P<0.01 or<0.05); however, for ET-1 of 2 μg/mL, the situation was the opposite. Moreover, after 24-hour culture with ET-1 of 0.2 μg/mL, the metabolic activity, cell adhesion rate, and expression of ICAM-1 peaked at 0.327±0.009, (163.31±4.05)% and 4.667±0.551, respectively. Conclusion ET-1 may enhance cellular metabolism and pigmentation by suppressing the expression of ICAM-1 and promoting the proliferation, adhesion and migration of melanoma cells.