载脂蛋白A—I基因启动子区域G→A置换对血浆HDL水平的影响

采用ＰＣＲ扩增法和限制性片段长度多态性分析法，对１１５例正常人和１１８例冠心病患者的载脂蛋白Ａ－Ｉ基因启动子区域－７８ｂｐ位点腺嘌呤与鸟嘌呤置换进行检测，并观察此位点基因变异对血浆ＨＤＬ－ｃｈ和ａｐｏＡ－Ｉ含量的影响。结果显示，血浆ＨＤＬ－ｃｈ和ａｐｏＡ－Ｉ含量在三种基因型中无显著性差异（Ｐ〉０．０５），基因型在正常ＨＤＬ－ｃｈ和低ＨＤＬ－ｃｈ水平中的分布差异亦无显著性（Ｐ〉０．０５）。结论：ａｐ

Genomic Analysis of the Substitution of G to A at Apolipoprotein Al Gene Promoter: Effects on Plasma HDL-Cholesterol

118 patients with coronary heart disease (CHD) and 115 control subjects were investigated in order to elucidate the association between the G to A substitution at -78bp in the promoter of apoAI gene and plasma HDL levels. ApoAI genotype was determined by polymerase chain reaction(PCR) amplification of genomic DNA and restriction with MspI. The results showed that plasma concentrations of HDL-ch and apoAI did not vary in different genotypes. The genotype frequencies in normal level of HDL did not differ from those in lower level. The analysis indicated that the mutation at -78bp did not affect the plasma HDL concentration, there was no association between A allele and high HDL level.