黑胸大蠊不同发育阶段可溶性蛋白抗原的分析

目的 了解黑胸大蠊的不同发育阶段蛋白质抗原免疫生化特性。方法 采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)对黑胸大蠊不同发育阶段可溶性蛋白质组分进行分离鉴定并通过酶联免疫印迹(ELIB)技术分析不同发育阶段抗原的免疫学特性。结果 四种抗原蛋白经SDS-PAGE后银染色，均得到清晰的蛋白显色带。卵抗原、若虫抗原、雄成虫抗原、雌成虫抗原分别可见13、28、26、41条蛋白区带，其中主带分别为2、10、10、13条，分子量大多位于10～97kDa范围。具有免疫原性的蛋白质大多分布在43kDa以上分子量范围，四种抗原组分相互之间有交叉抗原的存在。结论 不同发育阶段黑胸大蠊的蛋白质组分从卵．若虫．成虫出现次第增多现象并趋于复杂化，这对研究黑胸大蠊的发育生物学特征具有一定的潜在意义。

Study on the Soluble Protein Antigens Derived from Four Developmental Stages of Periplaneta fuliginosa

Objective To provide experimental data for further studies on the immunobiochemical characteristics of the protein antigens from Periplaneta fuliginosa at different developmental stages. Method Four groups of soluble protein antigens of the P. filiginosa, namely, egg antigens, nymph antigens, male and female adult antigens, were analyzed with SDS-PAGE and ELIB. Results Stripes of the studied antigens were clearly displayed by silver staining after SDS-PAGE. The egg antigens, nymph antigens, male and female adult antigens revealed 13, 28, 26 and 41 bands,respectively, of which 2, 10, 10 and 13 major bands were found in the respective groups. Most protein components varied in a molecular weight range of 10 ～ 97kDa. The ELIB analysis showed that proteins with immunogenic activity were mainly distributed in a molecular weight range of higher than 43kDa. The ELIB result also showed an existence of common antigenic components among these groups. Conclusions With the development of the cockroach from egg to adult, the P. fuliginosa protein components are increasingly aggrandized in number and complicated in composition. This observation is significantly contribute to the further studies on the biological features of P. filiginosa development.