植物次生物质和杀虫剂对分月扇舟蛾谷胱甘肽S-转移酶的抑制作用

本论文采用分光光度计法研究了植物次生物质和杀虫剂对分月扇舟蛾Closteraanastomosis(L.)谷胱甘肽S-转移酶(GSTs)的体外抑制作用。结果表明各植物次生物质和杀虫剂对分月扇舟蛾GSTs活性的体外抑制作用存在差异。当植物次生物质和杀虫剂终浓度为8.34×10-5mol/L时,槲皮素和单宁酸对分月扇舟蛾GSTs活性抑制作用最强,分别为64.41%和58.61%;三唑磷、毒死蜱、辛硫磷、氟铃脲、氟虫腈和哒螨灵都有较强的抑制作用,对GSTs活性抑制率超过20%;氧化乐果、水胺硫磷、丙溴磷、马拉硫磷、灭多威、联苯菊酯、高效氯氰菊酯、甲氰菊酯、高效氯氟氰菊酯、吡虫啉和啶虫脒对分月扇舟蛾GSTs有中等抑制作用;其他杀虫剂对分月扇舟蛾GSTs有较弱(或没有)抑制作用。此外,在一定的浓度范围内,槲皮素、单宁酸和辛硫磷对分月扇舟蛾GSTs活性的抑制作用存在明显的剂量效应关系。槲皮素、单宁酸和辛硫磷对分月扇舟蛾GSTs的抑制中浓度分别为2.19×10-5,2.62×10-5,1.49×10-4mol/L。因此,此研究明确了槲皮素和单宁酸作为防治分月扇舟蛾的新型防治剂或增效剂具有很好的潜力。

Inhibition of glutathione S-transferase activity by insecticides and allelochemicals in Clostera anastomosis

A biochemical investigation was made into the inhibition of glutathione S-transferase （GST） activity in Clostera anastomosis （ L. ） by insecticides and alleloehemicals. The results showed that different inhibitors have different effects on the inhibition of GST activity. When the final concentration of insecticides and allelochemicals was 8.34×10^-5 moL/L, quercetin and tannic acid were the most potent inhibitors tested, with inhibition percentages of GSTs in C. anastomosis of 64.41% and 58.61%. Among the inhibitors tested, triazophos, chlorpyrifos, phoxim, hexaflumuron, fipronil and pyridaben were inhibitory and omethoate, isocarbophos, profenofos, malathion, methomyl, bifenthrin, beta-cypermethrin, fenpropathrin, lambda-cyhalothrin, imidacloprid and acetamiprid were moderately inhibitory. Other insecticides were less inhibitory. Furthermore, quercetin, tannic acid and phoxim inhibited GST activity in vitro in a dose-dependent manner. The/5o values of these three inhibitors for GST activity in C. anastomosis larvae were 2. 19 ×10^-7 mol/L, 2. 53×10^-7 mol/L and 0. 15 ×10^-7 mol/L, respectively. The results indicate that quercetin and tannic acid have good potential to control C. anastomosis.