| 鉴定马铃薯染色体数目的方法研究 |
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| 引用本文： | 王舰,杨永智,蒲秀琴.鉴定马铃薯染色体数目的方法研究[J].黑龙江农业科学,2009(1):1-2. |
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| 作者姓名： | 王舰 杨永智 蒲秀琴 |
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| 作者单位： | 青海省农林科学院生物所,青海西宁,810016;青藏高原生物技术教育部重点实验室,青海西宁,810016 |
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| 摘 要： | 用块茎根尖、试管苗根尖及组培移栽苗匍匐茎尖为材料鉴定马铃薯的倍性，结果表明：利用匍匐茎尖作为材料，用饱和的对二氯溶液处理10-12h后，用改良的卡洛氏固定液固定24h，用1M的HCl60℃解离10-15min，进行染色15-20min，效果很好，表明在不易取材或材料稀少的情况下，用匍匐茎尖制片是一个鉴定马铃薯染色体数的有效经济的方法。
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| 关 键 词： | 马铃薯 种质资源 倍性鉴定 |
Study on Identified Method of Chromosome Count of Potato |
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| WANG Jian,YANG Yong-zhi,PU xiu-qin.Study on Identified Method of Chromosome Count of Potato[J].Heilongjiang Agricultural Science,2009(1):1-2. |
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| Authors: | WANG Jian YANG Yong-zhi PU xiu-qin |
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| Affiliation: | 1.Biology Institute of Qinghai Academy of Agricultural and Forestry Sciences;Xining;Qinghai 810016;2.Key Laboratory of Biotechnology in Qingzang Plateau;Ministry of Education;Qinghai 810016 |
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| Abstract: | The potato-ploidy was identified from the creeping rootstock,foot tip and foot tip of tissue culture respectively. These issue rootstocks were treated by Colchicine,8-Hydroxyquinoline, saturated 1,4-dichlorobenzene and ice-water for 12h,fixed by improved Cacnoy for 24 h,later dispelled for 10-15 min, stained by Carbol fuehsin 15-20 rain, the results showed that the method that creeping rootstock was better material,it could be treated better by 1,4-diehlorobenzene When there had many germplasm resources need to identify but rare material was better. |
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| Keywords: | potato germplasm resource identify ploidy |
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