Functional expression of dihydropyridine-insensitive calcium channels during PC12 cell differentiation by nerve growth factor (NGF), oncogenic ras,or src tyrosine kinase |
| |
Authors: | Dianne M Rausch Deborah L Lewis Jeffrey L Barker Lee E Eiden |
| |
Affiliation: | Unit on Molecular and Cellular Neurobiology, National Institute of Mental Health, NIH, Bethesda, Maryland 20892. |
| |
Abstract: | 1. Recombinant retroviruses were used to introduce a temperature-sensitive v-src gene and oncogenic c-Ha-ras into PC12 cells, and stable cell lines expressing these genes were established. 2. As previously reported, expression of v-src (Alema et al., 1985) or c-Ha-ras (Noda et al., 1985) in PC12 cells results in neurite outgrowth resembling that induced by NGF. We report here that v-src but not oncogenic c-Ha-ras induces a stable morphologic neuronal differentiation similar to treatment with NGF. Oncogenic c-Ha-ras-induced neurite outgrowth is not stable with long-term culture, rather the cells revert to an undifferentiated morphology with altered cell cycle kinetics. 3. The stable neuronal phenotype induced by v-src and NGF is characterized by the functional expression of dihydropyridine-insensitive calcium currents. |
| |
Keywords: | oncogene v-src c-Ha-ras PC12 neural differentiation calcium channel |
本文献已被 SpringerLink 等数据库收录! |
|