| 高效液相色谱法测定奥拉西坦氯化钠注射液中主药的含量 |
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| 引用本文： | 闫小燕,胡欣,曹国颖,费小非.高效液相色谱法测定奥拉西坦氯化钠注射液中主药的含量[J].中国药房,2006,17(8):618-620. |
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| 作者姓名： | 闫小燕 胡欣 曹国颖 费小非 |
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| 作者单位： | 1. 卫生部北京医院药学部,北京市,100730
2. 中国医学科学院肿瘤医院药剂科,北京市,100021 |
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| 摘 要： | 目的:建立以高效液相色谱法测定奥拉西坦氯化钠注射液中主药含量的方法。方法:色谱柱为IntersilCN-3,流动相为乙腈-水(6∶94),检测波长为214nm,流速为0.6ml/min,进样量为5μl,温度为室温。结果:奥拉西坦检测浓度在0.5625～288μg/ml范围内与峰面积积分值线性关系良好(r=0.9999);平均回收率为99.95%(RSD=1.36%)。结论:本方法准确、简便、专属性强、重现性好,可用于本品的含量测定与质量控制。
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| 关 键 词： | 高效液相色谱法 奥拉西坦氯化钠注射液 含量 |
| 文章编号： | 1001-0408(2006)08-0618-03 |
| 收稿时间： | 05 26 2005 12:00AM |
| 修稿时间： | 07 19 2005 12:00AM |
Determination of Principal Agent in Oxiracetam Sodium Chloride Injection by HPLC |
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| YAN Xiaoyan,HU Xin,CAO Guoying,FEI Xiaofei.Determination of Principal Agent in Oxiracetam Sodium Chloride Injection by HPLC[J].China Pharmacy,2006,17(8):618-620. |
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| Authors: | YAN Xiaoyan HU Xin CAO Guoying FEI Xiaofei |
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| Affiliation: | Dept.of Pharmacy, Beijing Hospital, Ministry of Health, Beijing 100730, China;Dept .of Pharmacy,Cancer Hospital,Chinese Academy of Medical Sciences,Beijing 100021,China |
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| Abstract: | OBJECTIVE:To develop a HPLC method for the assaying of the principal agent in oxiracetam sodium chloride injection.METHODS:The separation was performed on Intersil CN-3column;the mobile phase was a mixture of acetonitri_ le-water(6∶94)with detection wavelength at214nm,flow rate at0.6ml/min and sample size at5μl under room temperature. RESULTS:The linear correlation of oxiracetam was observed over the concentrations of0.5625to288μg/ml(r=0.9999).The average recovery was99.95%(RSD=1.36%).CONCLUSION:The method is accurate,convenient specific and repro-ducible,and suitable for the determination and quality control of Oxiracetam. |
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| Keywords: | HPLC Oxiracetam sodium chloride injection Contents |
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