白色假丝酵母菌对常用消毒剂抗力的观察

目的了解常用3种消毒剂聚维酮碘、手消毒剂、氯氧三嗪(三氯异氰尿酸)泡腾片对医院感染白色假丝酵母菌与标准菌株的杀灭效果是否存在差异,临床株对这3种消毒剂是否产生抗力。方法用肉汤稀释法进行最低抑菌浓度(MIC)及最小杀菌浓度(MBC)测定,采用悬液定量杀菌试验了解消毒剂对细菌的杀灭率。结果聚维酮碘对8株临床分离的白色假丝酵母菌和标准株的MIC、MBC有所不同,而手消毒剂、氯氧三嗪泡腾片则差异无统计学意义;100mg/L聚维酮碘、1/16手消毒剂、250mg/L氯氧三嗪泡腾片对标准株和8株临床株作用10min的杀灭率均达100%。结论 8株临床分离的白色假丝酵母菌对聚维酮碘的抗力有所不同,但未检测到对手消毒剂、氯氧三嗪泡腾片产生抗力的临床株;在临床应用中,适当的延长消毒剂的作用时间可以达到杀灭白色假丝酵母菌的效果。

Resistance of Candida albicans to Three Kinds of Commonly Used Disinfectants

OBJECTIVE To understand the killing effect of the three kinds of commonly used disinfectants povidone iodine (Iodophor),hand disinfectants,and symclosene(trichloroisocyanuric acid)effervescent tablets on the standard strains of Candida albicans strains,their differences and the resistance.METHoDS The broth dilution method was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) and the suspension quantitative bactericidal test was used to understand the killing rate of the disinfectants.RESULTS On the eight clinical isolates and the standard strains of C.alblcans the results of povidone iodine were different but the hand disinfectants,and the symelosene effervescent tablets were without difference in MIC and MBC.The 10 min killing rate of 100 mg/L povidoneiodine,1/16 hand disinfectant,and 250 mg/L symclosene effervescent tablets to the standard strain and eight clinical strains was all 100%. CONCLUSIONS The resistance of eight clinical isolates of C.albicans to the povidoneiodine may be different,but in this experiment we don't detecting the resistance of clinical strains to the hand disinfectants and symelosene effervescent tablets.In clinical applications,adequatedly lengthening the action time of disinfectants can achieve the effect on killing C.albicans,thus reducing hospital infections,and protecting the medical staff and patients.