白骨壤果实抗菌活性部位筛选及低极性成分GC-MS分析

【目的】研究白骨壤Avicennia mavina(Forsk.)Vierh.果实的抗菌活性部位及其有效成分。【方法】以6株致病菌为抗菌活性部位筛选模型,采用有机溶剂萃取法将白骨壤果实的乙醇提取物分为石油醚相、乙酸乙酯相、正丁醇相和水相等不同极性部位,通过测定抑菌圈直径(Diameter of inhitition zone,DIZ)和最小杀菌浓度(Minimum bactericidal concentration,MBC)进行抗菌活性研究,并采用GC-MS技术对经过甲酯化后的石油醚相进行化学成分分析。【结果】白骨壤果实乙醇提取物的石油醚相对金黄色葡萄球菌、蜡状芽孢杆菌和藤黄八叠球菌3株指示菌株表现出一定的抑菌活性;从白骨壤果实石油醚相中鉴定出19个化合物,主要为脂肪类,均为首次从白骨壤果实中鉴定出。【结论】白骨壤果实石油醚相具有良好的抗菌活性,其中的脂肪酸类是主要抗菌活性物质。

Antibacterial Activities Evaluation and GC-MS Analysis of the Low Polar Components from the Fruits of Avicennia mavina (Forsk) Vierh

Objective]The antibacterial fractions and constituents of the fruits of Avicennia mavina were screened.Methods]The antibacterial activities of the ethanolic extract and or-ganic fractions of the fruits of Avicennia mavina from the South China Sea were evaluated by diameter of inhitition zone (DIZ)and the minimum bactericidal concentration (MBC). And the low-polar compounds in the petroleum ether fraction were also analysed by GC-MS after methyl esterification.Results]The petroleum ether fraction showed antibacterial activi-ty on Staphylococcus aureus,Bacillus cereus and Micrococcus luteus.And 19 compounds were firstly identified from the fruits of Avicennia mavina by GC-MS,most of which were lipids.Conclusion]It was proposed that lipids should be the main antibacterial sub-stances in the petroleum ether fraction of the fruits of Avicennia mavina.