广西甘蔗鞭黑粉菌遗传多样性分析

【目的】分析广西甘蔗主产区甘蔗鞭黑粉菌遗传多样性水平,为研究病菌毒力变化及甘蔗病害综合治理提供新思路。【方法】从广西各甘蔗主要产区采集甘蔗鞭黑粉菌样品,提取其中70份单倍体菌株基因组DNA,使用优化的随机扩增多态性DNA标记(RAPD)反应体系和经筛选获得的RAPD引物进行PCR扩增,电泳谱带使用NTSYS2.1进行聚类分析,并构建系统发育进化树。【结果】使用筛选出的10个RAPD引物进行扩增,获得77条条带,每个引物扩增得到的条带数为4~11条,大小为150~3000 bp,包含6个多态性位点,群体多态位点百分率为7.79%。70个甘蔗鞭黑粉菌的相似系数为0.96~1.00,在0.96的遗传系数上菌株可分为两大类,其中类群1聚集了来自横县校椅镇的两个菌株,其余68个菌株分布在类群Ⅱ中。【结论】广西甘蔗鞭黑粉菌遗传分化度较低,片段多态性和菌株地理来源、寄主和交配型无显著相关性。

Genetic diversity of Sporisorium scitamineam in Guangxi

Objective]Genetic diversity of Sporisorium scitamineam from main sugarcane growing area of Guangxi was studied, in order to provide new ideas for comprehensive control of sugarcane smut. Method]S. scitamineam was collected from main sugarcane growing areas of Guangxi, and genomic DNA were extracted from 70 haploid strains. Optimized random amplified polymorphic DNA(RAPD) reaction system and 10 RAPD primers screened deliberately were used to de-tect polymorphism of S. scitamineam by PCR amplification. Then, according to electrophoretogram, Cluster analysis was conducted to construct phylogenic tree with NTSYS2.1 software. Result]The results showed that, 10 RAPD primer pair could amplify 4-11 bands each, with a total of 77 bands, each band ranged from 150 to 3000 bp in length, but only 6 polymorphic sites were detected, which accounted for a polymorphic ratio of 7.79% in population. The genetic similarity coefficient of 70 S. scitamineam strains ranged from 0.96 to 1.00, and those strains were clustered into 2 clusters at genet-ic similarity level of 0.96, the first cluster was comprised of 2 strains collected from Xiaoyi town of Hengxian county, while the remaining 68 stains were clustered into the second cluster. Conclusion]S. scitamineam is at low level of genetic differentiation in Guangxi. Polymorphism of S. scitamineam had no significant correlation with its geographical origin, hosts and mating types.