CCDC80对巨噬细胞源性泡沫细胞炎症因子表达的影响及机制

目的 探讨卷曲螺旋结合域蛋白80(CCDC80)对THP-1 巨噬细胞源性泡沫细胞炎症因子表达的影响及相关分子机制.方法 体外培养的THP-1 细胞用佛波酯(160 nmol/L)处理,诱导分化为巨噬细胞,然后使用氧化型低密度脂蛋白(50 mg/L)处理使其荷脂形成泡沫细胞,并进行常规细胞体外培养.ELISA 检测细胞...

The effect and mechanism of CCDC80 on the expression of inflammatory factors in macrophage-derived foam cells

Aim To investigate the effect of coiled-coil domain containing protein 80(CCDC80) on the expression of inflammatory factors in THP-1 macrophage-derived foam cells and related molecular mechanisms. Methods THP-1 cells were treated with phorbol ester(160 nmol/L) to induce the cells to differentiate into macrophages, and then treated with oxidized low density lipoprotein(50 mg/L) to form foam cell, performing conventional cell culture in vitro. The expression of interleukin-1β(IL-1β), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) and monocyte chemoattractant protein-1(MCP-1) was detected by ELISA, the expression of Toll like report 4(TLR4), lipoprotein lipase(LPL) and nuclear factor-κB(NF-κB) was detected by real-time PCR and Western blot. Foam cells were treated with TLR4 siRNA and LPL siRNA, and the effects of CCDC80 on the expression of TLR4, LPL, NF-κB and p-NF-κB in foam cells were detected by fluorescence quantitative PCR and Western blot. Results CCDC80 significantly increased THP-1 macrophage-derived foam cell inflammatory factor secretion and p-NF-κB expression. After TLR4 siRNA treated foam cells, the expression of TLR4, LPL and p-NF-κB reduced significantly. After LPL siRNA treated foam cells, the expression of LPL and p-NF-κB decreased significantly. Conclusion CCDC80 promotes the activation of NF-κB signaling pathway through the TLR4/LPL pathway, thereby increasing the secretion of IL-1β, IL-6, TNF-α and MCP-1.