| 解脲脲原体上转换发光免疫层析试纸条的制备 |
| |
| 引用本文： | 孙竞,郭建华,李蓓,周蕾,吴鹏.解脲脲原体上转换发光免疫层析试纸条的制备[J].郧阳医学院学报,2010,29(1):19-21. |
| |
| 作者姓名： | 孙竞 郭建华 李蓓 周蕾 吴鹏 |
| |
| 作者单位： | 孙竞,郭建华,吴鹏(陨阳医学院附属太和医院检验部,湖北 十堰,442000);李蓓(陨阳医学院临床病原学检验教研室,湖北 十堰,442000);周蕾(军事医学科学院微生物流行病研究所,北京,100071) |
| |
| 基金项目： | 湖北省教育厅重点项目 |
| |
| 摘 要： | 目的:获得抗解脲脲原体外膜多条带蛋白(MB蛋白)的多克隆抗体,制备能够用于解脲脲原体检测的上转换发光免疫层析试纸条。方法:以体外表达的解脲脲原体外膜MB蛋白N端保守性序列为抗原,免疫小鼠制备抗MB蛋白的抗体,ELISA检测免疫动物血清抗MB抗体滴度,并以小鼠抗体标记上转换发光体颗粒(UCP颗粒),制备能够检测解脲脲原体的上转换发光免疫层析试纸条。结果:解脲脲原体多条带蛋白免疫动物血清抗MB的IgG抗体滴度均超过1∶25600,以此抗体为基础建立的解脲脲原体上转换发光免疫层析试纸条能够识别1～200μg/L的解脲脲原体MB抗原。结论:初步制备的上转换发光免疫层析试纸条能够检测解脲脲原体。
|
| 关 键 词： | 解脲脲原体 外膜多条带蛋白 免疫血清 |
Establishment of UCL-immunochromatography Test Strip for Detecting MB Antigen of Ureaplasma Urealyticum |
| |
| SUN Jing,GUO Jian-hua,LI Bei,ZHOU Lei,WU Peng.Establishment of UCL-immunochromatography Test Strip for Detecting MB Antigen of Ureaplasma Urealyticum[J].Journal of Yunyang Medical College,2010,29(1):19-21. |
| |
| Authors: | SUN Jing GUO Jian-hua LI Bei ZHOU Lei WU Peng |
| |
| Affiliation: | SUN Jing1,GUO Jian-hua1,LI Bei2,ZHOU Lei3,WU Peng1 (1Department of Clinical Laboratory,Taihe Hospital,2Department of Microbiology,Yungyang Medical College,Shiyan,Hubei 442000,3Institute of Microbiology , Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China) |
| |
| Abstract: | Objective To obtain the polyclonal antibody against multiple banded(MB) antigen of ureaplasma urealyticum so as to establish Up-conversion luminescence-immunochromatography(UCL-immunochromatography) test strip of ureaplasma urealyticum.Methods The N-terminal conservative sequence of MB protein in vitro expressed was as antigen,antibody against MB antigen were prepared.The antibody titer of serum anti-MB were detected by ELISA and the UCL particles were labelled with mouse antibody.UCL-immunochromatography t... |
| |
| Keywords: | Ureaplasma urealyticum Multiple banded antigen Immune serum |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
